Lewis lung carcinoma cells

O Reilly M, Holmgren L, Shing Y, et al. Angiostatin a novel angiogenesis inhibitor that mediates the suppression of metastases by a Lewis lung carcinoma. Cell 1994 79 315-328. 

Thermosensitive block copolymer nanoparticles containing doxorubicin increased cytotoxicity against Lewis lung carcinoma cells when activated by heating above the LCST [139], Chitosan was chemically conjugated to NIPAAm/vinyl laurate copolymer to enhance gene transfection in mouse myoblast cells [140]. Upon i.v. administration, poly(NIPAAm) nanoparticles are taken up by the reticuloendothelial cells of the liver and mild inflammatory and fibrotic responses are observed... 

Adhesion to organ-specific structures can also be used to select metastatic variants, that will usually show increased homing to the tissue used for selection. In this way, B16 melanoma cells have been selected on lung cryostat sections (Netland and Zetter, 1981), Lewis lung carcinoma cells on hepatocyte monolayers (Brodt, 1989), and RAW117 large-cell lymphoma cells on hepatic sinusoidal cells (LaBiche et al., 1993). 

Plaksin, D., Gelber, C., Feldman, M. and Eisenbach, L. (1988). Reversal of the metastatic phenotype in Lewis lung carcinoma cells after transfection with syngeneic H-2Kb gene. Proc. Natl. Acad. Sci. USA 85, 4463-4467. 

Preparation and Characterization of STPP Liposomes for Animal Studies 1. PEG5000-PE (Avanti Polar Lipids, Alabaster, Alabama). 2. 5 mM HEPES-buffered saline, pH 7.4, prepared from HEPES (Fisher Scientific) and pH adjusted with I N HCl/1 N NaOH. 3. BalB/C mice (Charles river labs). 4. C57BL mice (Charles river labs). 5. LLC (Lewis Lung Carcinoma) cells (ATCC). 

C57BL mice with subcutaneous solid tumors generated by injecting LLC (Lewis Lung Carcinoma) cells are used for the biodistribution study (see Note 7). 

Solid murine tumor LLC (Lewis Lung Carcinoma) cell line is initiated in mice by a local subcutaneous injection. 2 x 10 cells in 0.2 cc of Hanks buffer salt solution are injected in each animal. Tumors are allowed to develop for 7-10 days after inoculation on reaching a surface diameter of 4-6 mm and a thickness of 2-3 mm. 

Cannabinoids have been recendy shown to reduce fhe number of metastatic nodes produced by paw injection of Lewis lung carcinoma cells in rats (Portella et al. 2003). Moreover, cannabinoid administration to glioma-bearing mice also decreases fhe activity and expression of matrix metalloproteinase-2 (MMP2), a proteolytic enzyme fhat allows tissue breakdown and remodelling during angiogenesis and metastasis (Blazquez et al. 2003). Hence it is conceivable that cannabinoids may also control tumour invasiveness. 

HA has been shown to produce synergistic therapeutic effects when coadministered with 5-fluorouracil (5-FU) and with PTX. HA formulated with 5-FU enhanced the cellular uptake and cytotoxicity of the drug compared to unformulated 5-FU (98). A similar effect has been observed when PTX was coadministered with HA (99). The admixture of PTX and HA significantly reduced the migration of Lewis lung carcinoma cells in a synergistic fashion and markedly improved the life span of mice seeded with tumor cells compared with PTX alone or HA alone. 

Dong, Z., Kumar, R., Yang, X., and Fidler, I. J. (1997) Macrophage-derived metalloelastase is responsible for the generation of angiostatin in Lewis lung carcinoma. Cell 88(6), 801-810. 

Viabilities of both B16 melanoma and Lewis lung carcinoma cells were decreased in the presence of S-nitroso-N-acetyl-DL-penicillamine in vitro and the cytotoxicity of S-nitroso-N-acetyl-DL-penicillamine was reduced dose-dependently by NO radical scavenger, oxyhemoglobin (Hirano 1997). Intravenous injection of both cell lines in mice exposed to 10-80 ppm NO gas did not reduce the tumour colony formation in the lung. The increase in NO concentration was accompanied by elevation of concomitant nitric dioxide concentration in exposure chambers and exposure to higher concentration of NO appeared to enhance tumour colony formation in the lung. 

O Reilly MS et al. (1994) Angiostatin a novel angiogenesis inhibitor that mediates the suppression of metastases by a Lewis lung carcinoma. Cell 79(2) 315-328 Overgaard J et al. (2005) Plasma osteopontin, hypoxia, and response to the hypoxia sensitiser nimorazole in radiotherapy of head and neck cancer results from the DAHANCA 5 randomised double-blind placebo-controlled trial. Lancet Oncol 6(10) 757-764... 

Heike Y, Takahashi M, Ohira T, et al. Genetic immunotherapy by intrapleural, intraperitoneal and subcutaneous injection of IL-2 gene-modified lewis lung carcinoma cells. Int J Cancer 1997 73 844-849. 

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