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Lateral-flow assay

O Farrell, B., and Bauer, J. (2006) Developing highly sensitive, more-reproducible lateral-flow assays. Part 1 New approaches to old problems. IVD Technol. 7(6), 41-50... [Pg.182]

Liposome-Enhanced Lateral-Flow Assays for the Sandwich-Hybridization Detection of RNA... [Pg.185]

Clinical and environmental analyses frequently necessitate rapid, simple, durable, and inexpensive point-of-care or field tests. Lateral-flow assays (LFAs), such as those used in pregnancy tests, often fulfill these requirements. These assays rely on the appearance of a colored band, the intensity of which corresponds to the concentration of the target analyte in the sample. The goal for... [Pg.185]

Assay Formats Two main assay formats are used in lateral-flow assays the competitive assay and the sandwich assay format. Both formats have been used for nucleic acid lateral flow assays, though the latter is more common (56, 58). The competitive assay format utilizes an analyte... [Pg.189]

Helbig, J. H., Luck, P. C., Kunz, B., and Bubert, A. (2006) Evaluation of the Duopath Legionella lateral flow assay for identification of Legionella pneumophila and Legionella species culture isolates. Appl Environ Microbiol. 72, 4489-4491... [Pg.212]

S., Feindt, FL, Niedbala, R. S., and Tanke, H. (2001) Use of up-converting phosphor reporters in lateral-flow assays to detect specific nucleic acid sequences a rapid, sensitive DNA test to identify human papillomavirus type 16 infection. Clin Chem. 47, 1885-1893... [Pg.213]

T. M. (2002) Qualification of cellulose nitrate membranes for lateral-flow assays. IVD Technol. [Pg.213]

Edwards, K. A., and Baeumner, A. J. (2006) Optimization of DNA-tagged dye-encapsulating liposomes for lateral-flow assays based on sandwich hybridization. Anal Bioanal Chem. 386,1335-1343... [Pg.213]

Chan, C. P, Sum, K. W., Cheung, K. Y., Glatz, J. F., Sanderson, J. E., Hempel, A., Lehmann, M., Renneberg, I., and Ren-neberg, R. (2003) Development of a quantitative lateral-flow assay for rapid detection of fatty acid-binding protein. J Immunol Methods. 279,91-100... [Pg.213]

Principles and characteristics of lateral flow strip assays are reviewed. Recent technology developments permit the use of inexpensive electronic readers for interrogating lateral flow strip test results, thus avoiding the inevitable variation and subjectivity of visual inspection to assess the capture of reporter-labeled analyte on test lines of the strip. Protocols for developing lateral flow assays are described, including two specific case studies for assaying cotinine (a small-molecule metabolite of nicotine) in a competitive format, and assaying HIV antibodies in a sandwich-type assay format. [Pg.217]

Key words Lateral flow assay, Rapid assay development, Nitrocellulose strips, Immunochromatography... [Pg.217]

Our laboratory has been using an Avagotech reader for evaluation of reagents for HIV and cotinine lateral flow assays. Figure 2. shows an example of results for a sample diluted in our prototype HIV antibody detection lateral flow test strips. The Avagotech reader allows the data to be plotted and coefficients of variation to be determined, making the screening more quantitative and less subjective. [Pg.221]

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See also in sourсe #XX -- [ Pg.17 ]



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