Laser-scanning cytometry

In addition to flow cytometers, cytometric instrumentation has evolved to include Laser Scanning Cytometry (LSC). The LSC is a cross between a flow cytometer and an imaging cytometer. Data are equivalent to the flow cytometer but is slide-based. Endpoints include light scatter and fluorescence, but the instrument can also record position of individual cells on the slide so that cells of interest can be relocated and re-evaluated or photographed. LSC is valuable when cell morphology is important. [Pg.118]

Note Cellular model was intestinal cells. The techniques employed for actin studies were laser-scanning cytometry and confocal microscopy... [Pg.113]

Smolewski P, Ruan Q, Vellon L, et al. Micronuclei assay by laser scanning cytometry. Cytometry. 2001 45(l) 19-26. [Pg.31]

Fasulo LM, Schomaker SJ, Ryan AM, et al. Comparison of biochemical analysis to gene expression and laser scanning cytometry for the assessment of rat hepatic cytochrome P450 (CYP) enzyme induction. Toxicol. Sci. (suppl.) 2009,108 56. [Pg.300]

Jones, D. L., Braiisford, M. A., and Drocourt, J.-L. Solid phase, laser-scanning cytometry a new two-hour method for the enumeration of microorganisms in pharmaceutical water. Pharm. Forum 25 7626—7645,1999. [Pg.304]

Baudart 1, Olaizola A, CoaUier 1, Gauthier V, Laurent P (2005) Assessment of a new technique combining a viabUity test, whole-ceU hybridization and laser-scanning cytometry for the direct counting of viable Enterobacteriaceae ceUs in drinking water. EEMS Microbiol Lett 243 405 09... [Pg.39]

D Haese E, Dumon I, Werbrouck H, Dejonghe V, Herman L (2005) Improved detection of Mycobacterium paratuberculosis in mfik. J Dairy Res 72 125-128 Guyomard S (1997) Vahdation of a scanning laser system for microbiological quality control (QC) analysis. Pharm Technol Fur (September) 50-54 Jones DL, Brailsford MA, Drocourt JL (1999) Solid-phase, laser-scanning cytometry a new two-hour method for the enumeration of microorganisms in pharmaceutical water. Pharmacopeia Forum 25 7626-7645... [Pg.40]

One advantage to examining apoptotic cells by laser-scanning cytometry is that subcellular structure may be examined. For instance, individual mitochondria may be examined for correlations among different parameters. Similarly, nuclear structure may also be examined in apoptotic cells. An example of the complimentarity of flow cytometry and CLSM is given in Fig. 4. TNF-a/ActD treated Hepa-1 cells were stained with CMX Rosamine... [Pg.25]

Bedner, E., Li, X., Kunicki, J., and Darzynkiewicz, Z. (2000) Translocation of bax to mitochondria during apoptosis measured by laser scanning cytometry. Cytometry 41(2), 83-88. [Pg.32]

Kamentsky, L. A. (2001) Laser scanning cytometry. Methods Cell Biol. 63, 51-87. [Pg.57]

Furuya, T., Kamada, T Murakami, T Kurose. A., and Sasaki, K. (1997) Laser scanning cytometry allows detection of cell death with morphological features of apoptosis in cells stained with PI. Cytometry 29, 173-177. [Pg.58]

Harnett, M. M. 2007. Laser scanning cytometry Understanding the immirne system in situ. Nat. Rev. Immunol. 7 897-904. [Pg.168]

Non-growth Based Method Solid State Laser Scanning Cytometry... [Pg.401]

Darzynkiewicz, Z., Smolewski, P., Holden, E., et al., 2011. Laser scanning cytometry for automation of the micronucleus assay. Mutagenesis 26,153—161. [Pg.673]

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