Laser ablation electrospray ionization LAESI

Laser ablation electrospray ionization (LAESI) is especially designed for biological samples containing water. Using this technique, a focused mid-IR laser excites the OH vibrations in a sample s water molecules. Phase explosion causes a rapid microscale ablation, ejecting a mixture of molecules, clusters, and particulate matter from the sample surface. Variations in the water content... [Pg.249]

Several other ionization methods have been developed based on DESI, including desorption atmospheric pressure chemical ionization (DAPCI), desorption atmospheric pressure photo-ionization (DAPPI), laser ablation electrospray ionization (LAESI), and extractive electrospray ionization (EESI). Each technique uses variations of the solvent, how the charged beam is formed, and how the beam is nsed to facilitate the prodnction of analyte ions. Because these are surface methods (except EESI), they are incompatible with LC. [Pg.65]

Probe electrospray ionization (PESI) and TOF-MS were used for direct profiling of phytochemicals in different parts of a fresh tulip bulb [80], which emphasized the possibility of conducting in-vivo MS analysis of less sensitive biological matrices such as plant tissues. Recently, Pan et al. [81] demonstrated single-probe MS which can conduct metabolomic analysis of individual living cells in real time. The diameter of this probe is < 10 pm which makes the device compatible with eukaryotic cells. Atmospheric pressure ion sources are particularly suitable for analysis of live biological specimens. Cellular metabolism does not need to be quenched before analysis. For instance, in laser ablation electrospray ionization (LAESI)-MS, cells are irradiated by a laser beam in order to extract small amounts of cytosolic components, and to transfer them to the ESI plume [82]. [Pg.329]

The ionization methods reported for IMS included MALDI [41,76-80], Secondary Ion Mass Spectrometry (SIMS) [19, 81-86], Matrix-enhanced (ME)-SIMS [87, 88], Desorption Electrospray Ionization (DESI) [89-99], Nanostructure Initiator Mass Spectrometry (NIMS) [100-102], Atmospheric Pressure Infrared MALDI Mass Spectrometry (AP-IR-MALDI-MS) [103], Laser Ablation-inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS) [104-106], Laser Desorption Postionization (LDPI) [107], Laser Ablation Electrospray Ionization Mass Spectrometry (LAESI) [108, 109], and Surface-assisted Laser Desorption/ioniza-tion Mass Spectrometry (SALDI) [110-112], Another method was called probe electrospray ionization (PESI) that was used for both liquid solution and the direct sampling on wet samples. [Pg.405]

HA-LAESI heat-assisted laser ablation electrospray ionization HR-EI high-resolution electron impact... [Pg.205]

LAESI Laser ablation electrospray ionization IR laser ablation with postionization by ESI plume [53]... [Pg.645]

Several other ionization methods and MS technologies have been investigated for possible use for imaging. Atmospheric pressure infrared MALDI (AP IR-MALDI) and mid-infrared laser ablation with electrospray ionization (LAESI) have been reported and use focused laser radiation for ambient sampling with no sample preparation or chemical modification required. In these... [Pg.12]