Lambda packaging

Lambda package extract (Promega Biotech or Stratagene) Sepharose 6-L spun column (5 -3 Inc.) y- P-ATP (5000 Ci/mmol, 10 mCi/ml, aqueous, Amersham) or P-dGTP (3000 Ci/mmol, 10 mCi/ml, aqueous, Amer sham)... 

Murialdo, H., 1991. Bacteriophage lambda DNA maturation and packaging. Annual Review of Biochemistry 60 125-153. Review of the biochemi.stry of packaging DNA into A phage heads. 

Casado JL, Perez-Ehas MJ, Marti-Belda P, Antela A, Suarez M, Ciancas E et al. (1998) Improved outcome of cytomegalovirus retinitis in AIDS patients after introduction of protease inhibitors. J Acquir Immune Defic Syndr Hum Retrovirol 19 130-134 Catalano CE (2000) The terminase enzyme from bacteriophage lambda a DNA-packaging machine, Cell Mol Life Sci 57 128-148... 

Cosmid A plasmid into which the DNA sequences from bacteriophage lambda that are necessary for the packaging of DNA (cos sites) have been inserted this permits the plasmid DNA to be packaged in vitro. 

The expert system package is designed to operate on a LISP machine interfaced with a conventional distributed control system. The design assumes that up to 20,000 measurement points and alarms may be accessed. The Lambda machine from LMI was utilized. The realtime data interface is via an integral Multibus connected to a computer gateway in the distributed system. 

In many cases the NMR fascilities in universities and industry are equipped with spectrometers manufactured by companies other than Bruker/Spectrospin. The NMR data from Varian (Gemini and Unity), JEOL (Alpha, GX, EX and Lambda) and GE spectrometers may also be processed by ID WIN-NMR and 2D WIN-NMR provided that the data is first converted into WIN-NMR data format. The data should be transferred to a PC, preferably via an internal network, and then the appropriate routine in the GETFILE module is used for the conversion. To use this conversion tools a copy protection dongle (WIBU-key) - not delivered with this education package - must be installed on your PC. If you plan to install this option, please refer to the instructions given in the GETFILE manual [2.3] and contact your Bruker/Spectrospin representative. 

Bacteriophage lambda (X) is a good example for considering phage infection. In lytic infection, the injected linear double-stranded X DNA first circularizes. It is then transcribed to produce viral proteins needed for viral DNA replication and packaging as well as many molecules of viral capsid proteins. The viral DNA is replicated and the DNA copies are packaged into new phage... 

Cosmid. A DNA molecule with cos ends from lambda-bacteriophage that can be packaged in vitro into a virus for infection purposes. ... 

The lambda phage method is readily adapted to the screening of M13 plaques (Mason and Williams, 1985). However, best results are obtained when the lifts are directly baked, without lysis and denaturation steps (M13 is 55). It is also advantageous to use ds probes or to prepare probes complementary to the strand packaged in M13. 

Gunther, E. J., Murray, N. E., and Glazer, P. M. (1993). High efficiency, restriction-deficient in vitro packaging extracts for bacteriophage lambda DNA using a new E. coli lysogen. Nucleic Acids Res 21, 3903-3904. 

---