Lambda bacteriophage

Bacterms Bacteriocide Bacteriocms Bacteriophage lambda Bacteriophages Bacteriorhodopsin... 

Fig. 4. Construction of recombinant phage in vectors derived from bacteriophage lambda where E represents the enzyme EcoRl. Other terms are defined...

Figure 8.1 A region of DNA in the related bacteriophages lambda, 434, and P22 that controls the switch for synthesis of new phage particles. Two structural genes are involved in this switch one coding for a repressor protein and one coding for the Cro protein. Between these genes there is an operator region (OR) that contains three protein binding sites—ORl, OR2, and OR3.

Table 8.1 The nucleotide sequences of the three protein-binding regions ORl, OR2, and OR3 of the operator of bacteriophage lambda...

Figure 8.3 The DNA-binding protein Cro from bacteriophage lambda contains 66 amino acid residues that fold into three a helices and three P strands, (a) A plot of the Ca positions of the first 62 residues of the polypeptide chain. The four C-terminal residues are not visible in the electron density map. (b) A schematic diagram of the subunit structure. a helices 2 and 3 that form the helix-turn-helix motif ate colored blue and red, respectively. The view is different from that in (a), [(a) Adapted from W.F. Anderson et al., Nature 290 754-758, 1981. (b) Adapted from D. Ohlendorf et al., /. Mol. Biol. 169 757-769, 1983.]...

Murialdo, H., 1991. Bacteriophage lambda DNA maturation and packaging. Annual Review of Biochemistry 60 125-153. Review of the biochemi.stry of packaging DNA into A phage heads. 

Casado JL, Perez-Ehas MJ, Marti-Belda P, Antela A, Suarez M, Ciancas E et al. (1998) Improved outcome of cytomegalovirus retinitis in AIDS patients after introduction of protease inhibitors. J Acquir Immune Defic Syndr Hum Retrovirol 19 130-134 Catalano CE (2000) The terminase enzyme from bacteriophage lambda a DNA-packaging machine, Cell Mol Life Sci 57 128-148... 

The Genetic Switch of Bacteriophage Lambda ( ) Provides a Paradigm for Protein-DNA Interactions in Eukaryotic Cells... 

Cosmid A plasmid into which the DNA sequences from bacteriophage lambda that are necessary for the packaging of DNA (cos sites) have been inserted this permits the plasmid DNA to be packaged in vitro. 

Christensen, A. 2001. Bacteriophage lambda-based expression vectors. Molecular Biotechnology 17, 219-224. 

Wang,J., Hofnung, M., and Charbit, A. (2000). The C-terminal portion of the tail fiber protein of bacteriophage lambda is responsible for binding to LamB, its receptor at the surface of Escherichia coli K-12./. Bacteriol. 182, 508-512. 

In lysogenic cytlos. replication of the bacteriophage lambda occurs without lysis of the infected tell. 

The bacteriophage lambda (A) contains the genes coding for resistance to ampicillin and tetracycline... 

FIGURE 6.3 Construction of a brain-specific cDNA library. Messages (mRNA) are isolated from a tissue of interest. In this case, the target tissue is the brain. The mRNAs are converted to cDNA and each cDNA is inserted into the DNA of the bacteriophage lambda. The end result is a brain-specific cDNA library. 

Hershey, A. D., ed. (1971) The Bacteriophage Lambda, Cold Spring Harbor Lab, Cold Spring Harbor, New York... 

---