Preparing probe ink

There are many approaches by which to prepare a probe for immobilization to a substrate by printing. The exact nature of the buffer (ink) composition will depend upon the surface characteristics of the substrate and the surface 

Source plates may be prepared in advance and stored frozen, depending upon fhe probe ink stability. In any case, source plates should be brought to ambient temperature to assure that all components have redissolved. We recommend that source plates be centrifuged to remove any entrapped air bubbles from fhe bottoms of the wells. While manual preparation of source plafes is possible, the use of a robotic dispensing sysfem is highly recommended in order to avoid mistakes in placement of fhe probe inks in fhe wells and to avoid cross-contamination. The use of such a device will also allow more xmiform preparation of subsequent source plates. 

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