In situ hybridization reactions

Tissue microarray (TMA) TMA technology permits to arrange hundreds or thousands of tissue cores (probes), such as clinical biopsies or tumor samples, on a single slide, and then to analyze by a single immuno-staining or in situ hybridization reaction. 

The detection of kappa or lambda messenger RNA via an in situ hybridization reaction provides an alternative to the immunohistochemical detection of cytoplasmic kappa or lambda light chain (Fig. 6.4C and D). This technique allows for a much cleaner reaction product because it is only demonstrated where there is mRNA for kappa and lambda and not protein. Several immunohistochemical standards now provide an automated platform for the performance of this in situ hybridization reaction. This allows for this technique to enter routine performance and can be accomplished in approximately 5 hours. 

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