8-aminopyrene-l,3,6-trisulfonate

H. Suzuki, O. Muller, A. Guttman, and B. L. Karger, Analysis of l-aminopyrene-3,6,8-trisulfonate-derivatised oligosaccharides by CE with MALDI TOF MS, Anal. Chem., 69 (1997) 4554-4559. 

Guttman, A., Chen, F.A., Evangelista, R.A. and Cooke, N. (1996) High-resolution capillary gel electrophoresis of reducing oligosaccharides labeled with l-aminopyrene-3,6,8-trisulfonate. 

Suzuki, H. Mueller, O. Guttman, A. Karger, B. L. Analysis of l-aminopyrene-3,6,8-trisulfonate-derivatized ohgosaccharides by capillary electrophoresis with matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. Anal. Chem. 1997,69,4554-4559. 

Glycoprotein was digested using the peptide N-Glycosidase F (PNGase F) to release N-linked glycan, then labeled with 8-aminopyrene-l,3,6-trisulfonate (APTS). The detection used LIF with a argon-ion laser. 

Zhuang et al. [152] derivatized glycan samples with 8-aminopyrene-l,3,6-trisulfonic acid to get a charge for electrophoresis and a fluorescent label for detection. They analyzed glycan derivatives in the blood of cancer patients... 

Yoshinaka, Y., Ueda, Y., and Suzuki, S. Ion-pair chromatographic separation of glycoprotein-derived oligosaccharides as their 8-aminopyrene-l,3,6-trisulfonic acid derivatives./. Chromatogr. A. 2007, 1143,83-87. 

In complex matrices, the insufficient specificity at 200 nm and the low sensitivity of direct UV detection make the analysis of carbohydrates more difficult. Therefore, derivatization of carbohydrates with a suitable agents is still a preferred approach for the detection of monosaccharides and oligosaccharides. Derivatization agents like 2-aminopyridine, 8-aminonaphthalein-l,3,6-trisulfonate (ANTS) or 8-aminopyren-l, 3,6-trisulfonate... 

Figure 12.3 Molecular structure of protonated amine photoacids 1-aminopyrene (lAP), /V-methyl-1-aminopyrene (MAP), /V,/V-dimethyl-l-aminopyrene (DMAP), 8-aminopyrene-l,3,6-trisulfonate (APTS).

FIGURE 10.13 Microchip electropherograms of 8-aminopyrene-l,3,6-trisulfonate (APTS)-labeled N-linked oligosaccharides from ribonuclease B (A) and APTS-labeled oligosaccharide ladder (B). Experimental conditions Esep = 300 V/cm, 0.5% methyl cellulose in 20 ruM phosphate buffer (pH 6.66). (Reprinted from Dang, R, Journal of Chromatography A 1109, 141, 2006. With permission.)... 

AMINOPYRENE-l,3,6-TRISULFONIC ACID TRISODIUM SALT (APTS)... 

Other Names 8-Aminopyrene-l,3,6-trisulfonic acid, trisodium salt APTS 1,3,6-Pyrenetrisulfonic acid, 8-amino-, trisodium salt Trisodium 8-aminopyrene-l,3,6-trisulfonate... 

Dang, E Chen, Y Guo, Q. Analysis of saccharides as 8-aminopyrene-l,3,6-trisulfonate derivatives by capillary electrophoresis with laser-induced fluorescence detection. Fenxi Huaxue 2000, 28, 80-83. 

Aminopyrene-l,3,6-trisulfonic acid trisodium salt (APTS) 63... 

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