L1210 cells

Dysidazirine 130 was isolated by Ireland in 1988 from a Fijian sample of the marine sponge Dysidea fragilis [194] as the major lipophilic component of the sponge and was shown to possess cytotoxic activity against L1210 cells, as well as... 

Kim et al. (1987) showed that the prolonged retention time of Ara-C in the peritoneal cavity after intraperitoneal administration of the drug in liposomal form as discussed above resulted in better therapeutic effects on intraperitoneally inoculated L1210 cells, as compared to the free drug. The activity of intraperitoneally administered cDDP on Ehrlich ascites carcinoma in mice was increased after encapsulation in neutral liposomes (Sur et al., 1983). The in vivo studies revealed improved antitumor activity and a lower toxicity sifter administration of cDDP liposomes compared to free drug. 

The stability of [3, 5, 7,9-3H]folic acid (Fignre 10.10) was analyzed to determine whether the varied and conflicting resnlts regarding the characteristics of folate transport in L1210 cells conld be due to impurities in the labeled snbstrate [19]. The susceptibility of [3, 5, 7,9-3H]folic acid to decomposition during storage at -20°C... 

Ewig, R.A.C., and Kohn, K.W. (1977) DNA - protein cross-linking and DNA interstrand cross-linking by haloethylnitrosoureas in L1210 cells. Cancer Res. 38, 3197. 

The influence of photoexcited fullerene C60 on viability of thymocytes, EAC, and L1210 cells was studied after 24 h of incubation, considering the content of viable cells at incubation as 100% (Table 6.1). After irradiation of fullerenes C60 in the cell medium, significant decrease in the content of viable cells in the suspension of thymocytes was not registered, whilst the number of viable malignant cells decreased. Upon the presence of photoexcited fullerene C60 in incubation medium, the number of viable EAC cells decreased by 20%, and L1210 by 12%, while in the... 

In the case of photoexcited fullerenes C60 and fullerene C60-containing composites in incubation medium of thymocytes, the indexes of LPO did not alter compared to the control too (Fig. 6.2A). Upon incubation of EAC cells in the presence of photoexcited samples of fullerenes, the decrease in the content of diene conjugates by 35% in the presence of fullerene C60 and by 20% in the presence of fullerene C60-composite-1 and fullerene C60-composite-2 was observed (Fig. 6.2B). The presence of photoexcited samples of fullerenes in the suspension of L1210 cells influenced LPO indexes only in the presence of fullerene C60-composite-2, when the content of diene conjugates increased by 35% (Fig 6.2C). 

Fig. 6.2 Content of diene conjugates (% from control) in thymocytes (A), EAC (B), and L1210 cells (C) after 1 h of incubation in the presence of photoexcited samples of fullerenes (1 - fullerene C60, 2 - C60-composite-l, 3 - C60-composite-2). P < 0.05 compared to the control...

Upon the presence of the fullerenes C60 in incubation medium of thymocytes and L1210 cells, the content of Schiff bases did not alter compared to the control (Fig. 

C60-composite-l, and by 26% in the presence of fullerene C60-composite-2 compared to suspension of L1210 cells incubated without addition of the samples of fullerenes (Fig. 6.4b). 

For evaluation of influence of the samples of fullerenes C60 on total metabolic state of thymocytes, EAC, and L1210 cells we have used MTT test based on the reduction of MTT by reductase system that consists, in particular, of mitochondrial succinatedehydrogenase. 

The rate of MTT reduction was determined adding the agent to cell incubation medium on 0.5,2, and 5 h after irradiation of samples. In the case of non-irradiation of fiillerenes C60 in incubation medium, the indexes of total metabolic state of thymocytes, EAC, and L1210 cells did not alter compared to the control. 

As one may see from the data presented in Fig. 6.5a, upon the presence of photoexcited samples of fullerenes in incubation medium of thymocytes, no significant changes in the rate of MTT reduction were observed during 5 h. Upon the presence of photoexcited fullerene C60 in incubation medium of suspension of L1210 cells,... 

The cytotoxicities of okadaic acid as EC50 against the P388 and L1210 cell lines are 1.7 nano-molar and 17 nano-molar, respectively. Additionally, okadaic acid strongly inhibits protein serine/threonine phosphatase 1,2A,... 

L1210 cells implanted i.p.. i.p. treatment (15 mice per group) N3P3AZ0 was dissolved in 0.9% ... 

Although the unusual dimerized phosphorohydrazide thioate 468 was isolated from the marine fungus Lignincola laevis, it was postulated that this compound is a biotransformation product of an insecticide that was present in the seawater. However, attempts to locate a comparable structure through the chemical registry have been unsuccessful. This compound showed cytotoxicity against L1210 cell line at a level of 0.25 pg/ml level [358]. 

Zenebergh, A., Baurain, R., and Trouet, A. Cellular pharmacology of detorubicin and doxorubicin in L1210 cells. Eur. J. Cancer Clin. Oncol. 20(1) 115-121, 1984. 

P388 mouse leukemia at a dose of 100 iig/mouse/day without exhibiting any toxicity [266], 2,4-Disubstituted 1,3-selenazoles (164, 165), evaluated for their antitumor activity by determining their ability to inhibit proliferation of L1210 cells in vitro, exhibited appreciable activity, although the 1,3-selenazole (165) was less potent than the sulfur analog [267, 268],... 

The Suzuki—Miyaura reaction of protected 6-chloropurine and 2-amino-6-chloropurine bases and nucleosides with substituted phenylboronic acids led to the corresponding protected 6-(substituted phenyl)purine derivatives 6—9. Their deprotection yielded a series of substituted 6-phenylpurine bases and nucleosides 10—13. Significant cytostatic activity (IC50 0.25—20 /tmol/ L) in CCRF-CEM, HeLa, and L1210 cell lines was found for several 6-(4-X-substituted phenyl-purine ribonucleosides 12 (X = H, F, Cl, and OR), while the 6-phenylpurine and 2-amino-6-phenylpurine bases 10 and 11, as well as 2-amino-6-phenylpurine ribosides 13, were entirely inactive against these cell lines. 

The title substituted 6 phenylpurine bases and nucleosides 10—13, as well as some acetyl derivatives 8, were tested on their in vitro inhibition of the cell growth in the following cell cultures mouse leukemia L1210 cells (ATCC CCL 219) murine L929 cells (ATCC CCL 1) human cervix carcinoma HeLa S3 cells (ATCC CCL 2.2) and human T lymphoblastoid CCRF-CEM cell line (ATCC CCL 119). Only substituted 6-phenylpurine ribonucleosides 12 and their triacetates 8 exhibited significant activity in these assays (Table 1), while the bases 10 and 11, as well as the 2 amino 6 phenylpurine ribonucleosides 13, were entirely inactive. 

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