Leukaemia L1210 cells

Three cyclic octapeptides, patellamides A-C (21-23) were isolated from L. patella and cytotoxicity data for these compounds and for ulicyclamide (15) and ulithiacyclamide (16) against L1210 murine leukaemia cells and the human acute lymphoblastic leukaemia (ALL) cell line CEM were reported [52]. The structures of the patellamides were later reassigned on the basis of synthetic studies. The proposed structures of patellamides B (22) and C (23) were synthesised and the products were shown to differ from the natural products. This led to new structures being proposed [53,54]. Separate syntheses of... 

Three guaiane sesquiterpenes, the ester (4.13), lactaroviolin (4.3), and deterrol (4.7) showed a weak mutagenic activity in the Ames Salmonella assay (137). In addition, deterrol (4.7) exhibited moderate cytotoxicity towards Ehrlich ascitic tumor cells (EGA cells) and weak toxicity towards L1210 cells (lymphocytic leukaemia mouse) (137). Lactaroviolin (4.3) showed a weak inhibitory effect towards EGA cells, while the ester 4.13 did not impair either cell line at 50 Xg/ml (137). The Swedish authors pointed out that the greater toxicity of deterrol than of lactaroviolin was rather... 

C9H14N4O4 242.234 Antineoplastic nucleoside. Shows significant activity against mouse leukaemia L1210, human T-cell acute lymphoblastic leukaemia Molt 4F and HeLa cells in vitro. Log P -3.03 (calc). Resistant to cytidine deaminase. 

The methyl sulfide-containing alkaloids, varamines A (123) and B (124) were isolated from L. vareau. Their structures were determined by interpretation of NMR spectral data and by comparison with related alkaloids. The varamines were cytotoxic towards L1210 murine leukaemia cells with IC50 values of 0.03 and 0.05 Xg/mL, respectively [138], The varamines (123-124), lissoclins (110-111) and diplamine (112) all contain a methyl sulfide group linked to a pyridoacridine ring system [22],... 

Fujianmycins A and B were isolated from a Streptomyces species (IA-CAS-114) in Fujian, China [167], The structure of fujianmycin A (34a) may be regarded as the 2,3-hydrated form of (33a), and fujianmycin B (34b) as its methyl ether derivative. The structure of antibiotic PD-116740 (35), isolated from an unidentified actinomycete species (WP-4669) and possessing activity against L1210 leukaemia in vitro and HCT-8 human colon adenocarcinoma cell line [168], may be visualized as the 3-hydroxylated 8-methyl-5,6-diol derivative of (33a). All these antibiotics contain the characteristic 2-phenylnaphthalene structural feature. 

Flow cytometric analyses of L1210 leukaemia cells exposed to JM216 revealed that the drug caused a slowdown in the S-phase of the cell cycle followed by a G2 block [35]. Cell death primarily occurred through apoptosis. 

In the late 1980s, ulapualide A 23 was isolated from the egg masses of the nudibranch Hexabranchus sanguineus120 and was shown to inhibit the growth of Candida albicans and L1210 leukaemia cell proliferation. A molecular mechanics study initially suggested that the relative stereochemistry was related to that of the halichondramides and mycalamides,350 however, the... 

Advantage has been taken of the binding affinity of (III.162) and (III.163) for TS to prepare affinity columns for the purification of this enzyme. One laboratory [58] utilized a column of the A °-formyl derivative (III. 163) immobilized on aminoethyl-Sepharose to purify TS to electrophoretic homogeneity from L1210 leukaemia cells. A second group [59] prepared a similar column from the A °-methyl derivative (III. 162) and used it to purify TS from neonatal mouse liver. 

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