Kidney slices

In further experiments to determine the rate of PIP2 hydrolysis in these rabbit kidney slices, radiolabel was... 

Y. Lee, J. Yang, S.M. Rudich, R.J. Schreiner, and M.E. Meyerhoff, Improved planar amperometric nitric oxide sensor based on platinized platinum anode. 2. Direct real-time measurement of NO generated from porcine kidney slices in the presence of L-arginine, L-arginine polymers, and protamine. Anal. Chem. 76,545-551 (2004). 

Isolated perfused kidney Isolated tubules Renal cells Cell lines Primary cells Kidney slices Isolated organelles... 

It is contended that the renal slice technique measures primarily basolateral uptake of substrates or nephrotoxins, based on histological evidence of collapsed tubular lumens. This results in the inaccessibility of brush-border surfaces for reabsorptive transport (Burg and Orloff, 1969 Cohen and Kamm, 1976). This observation limits the ability of this model to accurately reflect reactions to nephrotoxins that occur as the result of brush-border accumulation of an injurious agent. Ultrastructurally, a number of alterations, particularly in the plasma membrane and mitochondrial compartments, have been shown to occur over a 2-h incubation period (Martel-Pelletier et al., 1977). This deterioration in morphology is very likely a consequence of the insufficient diffusion of oxygen, metabolic substrates, and waste products in the innermost regions of the kidney slice (Cohen and Kamm, 1976). Such factors also limit the use of slices in studying renal metabolism and transport functions. 

Cross, R.J. and Taggart, J.L. (1950). Renal tubular transport Accumlation ofp-aminohippurate by rabbit kidney slices. Amer. J. Physiol. 161 181-190. 

Figure 8. Galactose transport by kidney slices from adult vs. newborn...

Similar studies have been reported by Segal et al (85) on the late development of the sugar transport processes in kidney, as measured by the ability of kidney slices from newborn animals to accumulate a-methyl glucoside. The concentrative ability is first observed 5 days following birth and does not reach the adult levels until 25 days of age. 

A note on the rapid conversion of fevo-kynurenine to anthranilic acid, and the conjugation of the latter with glucuronic acid by riboflavin-deficient rat-liver and rat-kidney slices, has recently appeared.148... 

Kidney Uptake Blood Epithelial cells Kidney slices, isolated and cultured renal epithelial cells, basolateral membrane vesicles, transporter expressions system... 

Kidney slices have been widely used to characterize renal uptake. The extracellular marker compounds, such as methoxyinulin and sucrose, were below the limit of detection in the luminal space of the proximal tubules, while they could... 

Wedeen RP, Weiner B. The distribution of p-aminohippuric acid in rat kidney slices. I. Tubular localization. Kidney Int 1973 3 205-213. 

Nozaki Y, Kusuhara H, Kondo T, et al. Characterization of the uptake of OAT1 and OAT3 substrates by human kidney slices. J Pharmacol Exp Ther 2007 321 362-369. 

After its invention by Burg et al. (1966) this technique has been used successfully in the kidney tubule segments of several species man, rabbit, rat, mouse, hamster, snake, birds etc. The tubule segments are dissected from thin kidney slices (< 1 mm thickness). Usually dissection can be done using sharpened forceps or needles without the addition of proteases (collagenase). The segment is identified by its anatom-... 

Fisher RL, Shaughnessy RP, Jenkins PM et al. (1995) Dynamic organ culture is superior to multiwell plate culture for maintaining precision-cut tissue slices. 1. Optimization of the tissue slice culture. Toxicol Methods 5 99-113 Forster RP (1948) Use of thin kidney slices and isolated renal tubules for direct study of cellular transport kinetics. Science 108 65-67... 

Howard, C.F., and Anderson, L., 1967, Metabolism of myo-inositol in animals, complete catabolism of myo-inositol-14C by rat kidney slices. Arch. Biochem. Biophys. 118 332-339. 

Plasma Concentrations of Calcium and Phosphate Although the mean response to changes in plasma cedcium is a chemge in the secretion of peuathyroid hormone, the activity of calcidiol 1-hydroxyleise in kidney slices is decreased directly by high concentrations of cedcium in the incubation medium. Cedcium heis no direct effect on the activity of calcidiol 24-hydroxylase under these conditions. Strontium and cadmium edso inhibit ctdcidiol 1-hydroxylase. 

Kidney slices Easy technique. Good control of experimental conditions without concern for secondary effects due to hemodynamic changes. Functional status of tubular lumen not clear. Tissue not homogenous and contains nontubular elements. Diffusion barrier for substrates to nephrons beneath the cut surface. 

Nierenberg DW. Competitive inhibition of methotrexate accumulation in rabbit kidney slices by nonsteroidal anti-inflammatory drugs. J Pharmacol ExpTher 1983 226 1-6. 

Absorption of lithium from guinea pig jejunum has been studied using an isolated mucosal preparation from which the underlying mus-cularis mucosae has been stripped (164). Absorption was shown to occur via a paracellular route and was unaffected by metabolic inhibitors or antidepressant, neuroleptic, or antiinflammatory drugs (165-167). These findings confirmed earlier work using everted sacs prepared from rat intestine (168, 169). The passive nature of lithium uptake into both kidney slices and isolated guinea pig intestinal epithelial cells has also been reported (170, 171). 

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