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Islet activating protein

Figure 11. Effects of EGTA or treatment with islet activating protein (lAP or pertussis toxin) on the 6-HCH-induced Ca response detected in Indo-l-labeled cells. Cells were treated for 2 hours at 37 C with (lAP) or without (Control) 10 Jg/mL lAP, then labeled with Indo-1. Cells were washed and resuspended at 2 x 10 cells/mL buffer and stimulated with 6-HCH (solid trace). In some cases (dashed traces), stimulation was preceded by the addition of 5-mAf EGTA 10 s before stimulation. Other experimental conditions are as in Figure 9. Figure 11. Effects of EGTA or treatment with islet activating protein (lAP or pertussis toxin) on the 6-HCH-induced Ca response detected in Indo-l-labeled cells. Cells were treated for 2 hours at 37 C with (lAP) or without (Control) 10 Jg/mL lAP, then labeled with Indo-1. Cells were washed and resuspended at 2 x 10 cells/mL buffer and stimulated with 6-HCH (solid trace). In some cases (dashed traces), stimulation was preceded by the addition of 5-mAf EGTA 10 s before stimulation. Other experimental conditions are as in Figure 9.
Fig. 1. Mechanisms of activation and inactivation of adenylate cyclase. Abbreviations not given in the text or figure are cholera T, Vibrio cholerae toxin IAP, islet activating protein, a Bordetetla pertussis toxin guanine N, guanine nucleotide 0- and a2-agonists, 0- and a2-adrenergic agonists 0. and a, a subunits of Gs and Gj, respectively. Fig. 1. Mechanisms of activation and inactivation of adenylate cyclase. Abbreviations not given in the text or figure are cholera T, Vibrio cholerae toxin IAP, islet activating protein, a Bordetetla pertussis toxin guanine N, guanine nucleotide 0- and a2-agonists, 0- and a2-adrenergic agonists 0. and a, a subunits of Gs and Gj, respectively.
G-protein identification has been aided considerably by the ability of their a subunits to be specifically ADP-ribosylated by either cholera or pertussis toxins. Cholera toxin ribosylates both transducin and Gs, causing dissociation of the holomeric form of these G-proteins to release a permanently activated and ADP-ribosylated a subunit. In contrast, pertussis toxin (islet activating protein, IAP) ribosylates the a subunit of the holomeric forms of Gi G0, Gh and transducin preventing their dissociation and, in the case of Gj, blocking the action of inhibitory hormones on adenylate cyclase activity. In the case of Gj, G0 and transducin, it has been clearly demonstrated that pertussis toxin can only ribosylate the holomeric form of these G-proteins. Cholera toxin, however, can ADP-ribosylate the free a subunits of Gs and transducin equally well, if not better, than when they form part of the holomeric complex. [Pg.337]

Journot L, Homburger V, Pantaloni C, Priam M, Bockaert J, Enjalbert A (1987) An islet activating protein-sensitive G protein is involved in dopamine inhibition of angiotensin and thyrotropin-releasing hormone-stimulated inositol phosphate production in anterior pituitary cells. J Biol Chem 363 15106-15110. [Pg.144]

Bokoch GM, Katado T, Northup JK, et al. (1983) Identification of the predominant substrate for ADP-ribosylation by islet activating protein. In J. Biol. Chem. 258 2072-2075,... [Pg.12]

Katada T, Oinuma M, Ui M (1986) Two guanine nucleotide-binding proteins in rat brain serving as the specific substrate of islet-activating protein, pertussis toxin. Interaction of the a-subunits with 3y-subunits in development of their biological activities. In J. Biol. Chem. 261 8182-8191. [Pg.46]

Katada T, Ui M (1982) Direct modification of the membrane adenylate cyclase system by islet-activating protein due to ADP-ribosylation of a membrane protein. In Proc. Natl. Acad. Sci. USA 79 3129-3133. [Pg.46]

Moss J, Stanley SJ, Burns DL, etal. (1983) Activation by thiol of the latent NAD gly-cohydrolase and ADP-ribosyltransferase activities of Bordefella pertussis toxin (Islet activating protein). In J. Biol. Chem. 258 11879-11882. [Pg.47]

Tamura M, Nogimori K, Murai S, et a/. (1982) Subunit structure of islet-activating protein, pertussis toxin, in conformity with the A-B model. In Biochemistry 21 5516-5522. [Pg.48]

Ui, M (1984) Islet-activating protein, pertussis toxin a probe for functions of the inhibitory guanine nucleotide regulatory component of adenylate cyclase. In Trends Pharmacol Sci 5 277—279... [Pg.62]

Yajima M, Hosoda K, Kanbayashi Y etal. (1978a) Islets-activating protein (lAP) in Bordetella pertussis that potentiates insulin secretory responses of rats. In J Bio-chem (Tokyo) 83 295-303... [Pg.62]

Yajima M, Hosoda K, Kanbayashi Y et al. (1978b) Biological properties of islets-activating protein (lAP) purified from the culture medium of Bordetella pertussis. In J Biochem (Tokyo) 83 305-312... [Pg.62]

K. Aktories, G. Schultz, and K.H. Jakobs, Islet-activating protein prevents nicotinic acid-induced GTPase stimulation and GTP but not GTPS-induced adenylate cyclase inhibition in rat adipocytes, FEBS Lett., 1983, 156, 88-92. [Pg.325]

Endo, Y., Lee, M.A., and Kopf, G.S. (1988). Characterization of an islet-activating protein sensitive site in mouse sperm that is involved in the zona pellucida-induced acrosome reaction. Dev. Biol. 729 12-24. [Pg.224]

Nomura, Y., Kitamura, Y. and Segawa, T. (1985). Decrease of clonidine binding affinity to a2-adrenoceptor by ADP-ribosylation of 41,000-dalton proteins in rat cerebral cortical membranes by islet-activating protein. ]. Neurochem., 44, 364-369... [Pg.243]

Okajima, F. and Ui, M. (1984). ADP-ribosylation of the specific membrane protein by islet-activating protein, pertussis toxin, associated with inhibition of a chemotactic peptide-induced arachidonate release in neutrophils A possible role of the toxin substrate in Ca " -mobilizing biosignalling. ]. Biol Chem., 259, 13863-13871... [Pg.246]

Katada T, Tamura M, Ui M. The A protomer of islet-activating protein, pertussis toxin, as an active peptide catalyzing ADP-ribosylation of a membrane protein. Arch Biochem Biophys 1983 224(1 ) 290-298. [Pg.10]

ADP-Ribosylation of a Membrane Protein Catalyzed by Islet-Activating Protein, Pertussis Toxin... [Pg.551]

Katada T, Ui M (1979) Islet-activating protein. Enhanced insulin secretion and cyclic AMP accumulation in pancreatic islets due to activation of native calcium ionophores. J Biol Chem 254 469-479... [Pg.559]

Katada T, Ui M (1981) In vitro effects of islet-activating protein on cultured rat pancreatic islets. Enhancement of insulin secretion, cAMP accumulation and " Ca flux. J Biochem (Tokyo) 89 979-990... [Pg.559]

Katada T, Ui M (1981) Islet-activating protein. A modifier of receptor-mediated regulation of rat islet adenylate cyclase. J Biol Chem 256 8310-8317... [Pg.559]

Katada T, Amano T, Ui M (1982) Modulation by islet-activating protein of adenylate cyclase activity in C6 glioma cells. J Biol Chem 257 3739-3746... [Pg.559]

Murayama T, Katada T, Ui M (1983) Guanine nucleotide activation and inhibition of adenylate cyclase as modified by islet-activating protein, pertussis toxin, in mouse 3T3 fibroblasts. Arch Biochem Biophys 221 381-390... [Pg.559]

See other pages where Islet activating protein is mentioned: [Pg.42]    [Pg.1]    [Pg.338]    [Pg.49]    [Pg.317]    [Pg.121]    [Pg.147]    [Pg.715]    [Pg.381]    [Pg.244]    [Pg.246]    [Pg.551]    [Pg.552]    [Pg.559]   


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