Ipecac clonal propagation

Clonal propagation of ipecac through shoot tip culture (Method I)... 

Ipecac plants clonally propagated by the method provided in the section 1.1 were used for the experimental materials. The potted plants acclimatized in a phytotron [25°C, 16 hr/day light (200 pEm S ), 80% relative humidity] for 1 month were transferred to the field in May (1987-1988) either soon (IM-plant) or after 1-year pre-cultivation (1 Y-plant) in a greenhouse (20 to 35 °C) and then cultivated under cheese cloth (50% shading rate). Plants cultivated in the field and greenhouse of Tsukuba and Tanegashima were periodically collected to determine their growth and alkaloid contents. 

Plant biotechnological application is promising as an alternative source for the production of pharmaceuticals. Clonal propagation of ipecac plants through shoot meristem culture [section 1.1] adventitious shoot formation on in vitro intemodal segment [section 1.4] and on cultured... 

In the meantime, the formation of the main alkaloids in C. ipecacuanha under a variety of conditions has been extensively investigated emetine (1) in callus cultures (49) and under the effects of L-tyrosine supplementation (5t)) emetine (1) and cephaeline (2) in Panamanian ipecac (57), in Nicaraguan ipecac (52), in regenerates obtained by clonal propagation (53,54), in tissue cultures (55) and under the effects of exogenous feeding of shikimic acid and L-phenylalanine (55), in cell suspension and excised root cultures (57), in adventitious root cultures (58), and in callus cultures (56,59) and the effects of age and electrokinetic potential (60) ipecoside (7) in the roots (61) and the effect oi Azotobacter, leaf mold, and farmyard manure on alkaloid content (62). In addition, micropropagation systems for C. ipecacuanha have been developed (63-65). 

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