Intraperitoneal perfusion

Figure 7 shows the response of the redox potential in a perfused hamster liver to the addition of 45 mN ethanol. Instead of the vitro labeling strategy Just described. the pyridine nucleotide pools in this hamster liver were labeled vivo by intraperitoneal injection of 35 mg [5- C] nicotinamide 5 hours prior to sacrifice. The bottom two spectra (2.6 min and 12.8 min) were obtained prior to addition of ethanol. They show resonances from labeled NAD, natural abundance glycogen and natural abundance choline methyl groups of phospholipids but no resonance from reduced pyridine nucleotides. After addition of 45 mM 10% [1- C] ethanol (at 17.9 min), resonances from C-1 of ethanol and NADH are detectable. These data demonstrate that the pyridine nucleotide pools labeled by intraperitoneal injection are metabolically active and that addition of 45 mN ethanol results in a marked change in the redox potential of the liver as measured by NNR. Furthermore, the observation of separate resonances for the oxidized and reduced pyridine nucleotides indicates that chemical exchange between oxidized and reduced forms is slow on the NNR time scale, and demonstrate that NNR may be used to quantitate the redox potential of free pyridine nucleotides situ. 

Liver perfusion. Mice are anaesthetized by intraperitoneal injection of pentobarbital (0.07 mg/g bodyweight dilute pentobarbital stock solution 7x with 0.9% NaCl, and inject 0.1 ml/10 g bodyweight). Abdomen is opened, portal vein exposed and loosely tied off with sterile surgical thread. Portal vein is then cannulated with the blunt needle, which is tied securely in place with a knot. Inferior vena... 

Animals are anesthetized by intraperitoneal injection of 60 mg/ml pentobarbital or as in step 2. Extensive perfusion can lead to overfixation. 

Liver is the preferred tissue for UDS measurement because it is well-perfused and the main metabolic site for absorbed compounds (during first-pass of compounds administered by the oral and intraperitoneal routes). Moreover, it is a slow-dividing tissue, and only a small proportion of cells undergo replicative DNA synthesis. Therefore DNA synthesis in most liver cells is limited to DNA repair (Butterworth et al. 1987 Mirsalis and Butterworth 1980). 

D-Fructose can cause structural alterations of liver in 10 to 90 minutes after intraperitoneal injection. Dense plaques appear in 20% of the liver nucleoli, and the plaques become more prominent in 3 hours. However, a similar change is also seen in the same number of nucleoli when D-glucose is given.270 Administration of D-fructose by intravenous perfusion induces ultrastructural alterations in smooth membrane, proliferations, cytolysome formation, and hyaloplasmic rarefaction. However, ATP content must decrease by 50% or more before gross, structural changes are observed.270... 

The ability of combretastatin A-4 disodium phosphate to induce vascular damage and enhance the radiation response of murine tumors was investigated [97]. A C3H mouse mammary carcinoma transplanted in the foot of CD FI mice and the KHT mouse sarcoma growing in the legmuscleofC3H/HeJ mice were used. CA-4-DP was dissolved in saline and injected intraperitoneally. Tumor blood perfusion was... 

Rats were anaesthetised by intraperitoneal injection of 30 mg pentobarbital/kg and perfused from the abdominal aorta with 2.5 % glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.4), postfixed with 1 % osmium tetroxide in sodium cacodylate buffer and embedded in Epon 812. Sections cut at 50 nm were stained with lead citrate and uranyl acetate. 

Fig. 149. Intimate contact of the plasmalemmata of a mononuclear phagocyte (bottom) and a mast cell (top) in a mesenteric lymph node (block 4477) of a 215 g Sprague-Dawley rat (Charles River, France) injected intraperitoneally with 50 mg montmorillonite API H 26 suspended in 2 ml saline. 4 days later under pentobarbital anaesthesia (30 mg/kg), the animal was perfused from the abdominal aorta with 2.5 % glutaral-dehyde in 0.1 ml sodium cacodylate buffer (pH 7.4). Postfixation with 1 % osmium tetroxide in sodium cacodylate buffer. Embedded in Epon 812 and sectioned at 50 nm. Lead citrate and uranyl acetate. Film 271/79...

Fig. 196. Mast cells from a thoracic lymph node (block 4471) of a 224 g Sprague-Dawley rat (Charles River, France) injected intraperitoneally with 50 mg montmorillonite API H26 suspended in 2 ml saline. 4 days later under pentobarbital anaesthesia (30 mg/kg), the animal was perfused from the...

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