Intemucleosomal DNA Fragmentation

Boidier, C., and Laurent, G., 1996, Daunombicin-induced intemucleosomal DNA fragmentation in acute myeloid cell lines. Leukemia 10 417-425. 

Bhalla K, Ibrado AM, Tourkina E, et al. Taxol induces intemucleosomal DNA fragmentation associated with programmed cell death in human myeloid leukemia cells. Leukemia 1993 7 563-568. 

Gl. Garcia-Martinez, V., Macias, D., Ganan, Y, Garcia-Lobo, J. M., Francia, M. V., Fernandez-Teran, M. A., and Hurle, J. M., Intemucleosomal DNA fragmentation and programmed cell death (apoptosis) in the interdigital tissue of the embryonic chick leg bud. J. Cell Set 106, 201-208 (1993). 

Flg.(6). Morphological appearance of PC-12 cells treated for 24 h with vehicle alone (panel A), TNF-a (500 units/ml) alone (panel B), or TNF-a (500 units/ml) plus 10 pM crocin (panel C). In panel D, intemucleosomal DNA fragmentation was detected by agarose gel electrophoresis of DNA extracted from cells treated for 24 h. 

We have previously shown that 4-hydroxynonenal (4-HNE) induced oxidative stress and apoptotic cell death in Swiss 3T3 fibroblasts. Indeed, in vitro treatment of 3T3 fibroblasts with 4-HNE induced a condition of oxidative stress as monitored by the oxidation of dichlorofluorescein diacetate. Further, 4-HNE-treated fibroblasts eventually underwent apoptotic death as determined by differential staining and intemucleosomal DNA fragmentation. These observations are consistent with a potential role of lipid peroxidation-derived products in programmed cell death. " ... 

Caspase-3 Activation, Cleavs of DFF45> and Degradation of DNA into 50-kb Fragments Are Insufficient for Induction of Intemucleosomal DNA Fragmentation in Etoposide-Treated Osteosarcoma Cells... 

Intemucleosomal DNA fragmentation is the result of activation of the endogenous endonuclease in apoptotic cells. The traditional gel electrophoresis method for the analysis of DNA fragmentation is labor-intensive. However, a microfluidic device has been developed which integrates the selection of individual cells, the lysis of cells, and the separation of the released apoptotic DNA fragments. 

Apoptotic cells are characterized by a distinct set of morphological and biochemical characteristics observed exclusively with this mode of cell death. These apoptotic features include loss of cell volume or cell shrinkage, chromatic condensation, intemucleosomal DNA fragmentation, and the formation of apoptotic bodies that are... 

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