Integrating Strategy as General Scale-Up Concept in Bioprocessing

The amount of carbon source is adjusted in such a manner that only one doubling in biomass can take place, which means that the sugar must be consumed after the time In 2//t. 

The time period with no growth must be sufficient that x again is brought to Xq, which means that 

The total time between two additions of carbon source is therefore 

This system will give a continuous synchronous culture in which the cell growth rate is independent of dilution rate (In 2/D) as long as D . A new generation of cells is produced every In 2/D. 

For microbial biomass production phased cultures would appear to have a 50% production advantage by permitting the culture volume to be harvested at doubling time intervals, rather than at replacement or retention time intervals (which correspond to approximately 1.5 times the doubling time). 

---

Integrating Strategy as General Scale-Up Concept in Bioprocessing 381... 

---