Inorganic Phosphate Transporters

Uchino, H., et al. Faropenem transport across the renal epithelial luminal membrane via inorganic phosphate transporter Nptl. Antimicrob. Agents Chemother. 2000,... 

Bellocchio EE, Reimer RJ, Fremeau RT, Jr., Edwards RH (2000) Uptake of glutamate into synaptic vesicles by an inorganic phosphate transporter [see comments]. Science 289 957-960. 

Uchino Ei, Tamai I, Yamashita K, Minemoto Y, Sai Y, Yabuuchi Ei, Miyamoto K, Takeda E, Tsuji A. p-aminohippuric acid transport at renal apical membrane mediated by human inorganic phosphate transporter NPT1. Biochem Biophys Res Comm 2000 270 254-259. 

The type El sodium-dependent phosphate (Na/Pi) transporters involved in mineralization are members of the inorganic phosphate transporter (PiT) family, which is conserved in all biology. El osteoclasts, a proton (H+) gradient instead of a sodium (Na+) gradient transports Pi. These transporters are antiports the Na+ or H+ is transported out as the Pi is transported in (Sects. 2.2.3 and 10.1.4). All these transporters are composed of repeating alpha helices that weave in and out of a membrane with intra- and extracellular turns (Fig. 9.7). A similar kind of structure is proposed for the annexins that mediate calcium ion transport. 

Uchino, H., Tamai, K.. Yamashita, K.. Minemoto, Y, Sai, Y, Yabuuchi, H.. Miyamoto, K., Takeda, E., and Tsuji. A. (2000) p-Aminohippuric add transport at renal apical membrane mediated by human inorganic phosphate transporter NPTl. Biochemical and Biophysical Research Communications, 270, 254—259. 

Willsky, C.R. and Malamy, M.H. (1980) Characterization of two genetically separable inorganic phosphate transport systems in Escherichia coli. Journal of Bacteriology 144, 356-365. 

GR WiUsky, MH Malamy. Effect of arsenate on inorganic phosphate transport in Escherichia coll J Bacteriol 144 366-374, 1980. 

The sodium-dependent inorganic phosphate transporter SLC34A1 (NaPi-lIa) is not localized in the mouse brain a case of tissue-specific antigenic cross-reactivity. J Histochem Cytochem 59 807-812... 

CycHc adenosine monophosphate (cAMP), produced from ATP, is involved in a large number of ceUular reactions including glycogenolysis, Hpolysis, active transport of amino acids, and synthesis of protein (40). Inorganic phosphate ions are involved in controlling the pH of blood (41). The principal anion of interceUular fluid is HP (Pig. 3) (41). 

Substitution of Asn or Glu for Asp707 inhibited Ca transport both with ATP or acetylphosphate as energy donors the formation of E P from ATP in the presence of 0.1 mM Ca or from Pi in the absence of Ca was also inhibited [103]. This data is consistent with a requirement for Asp707 in the phosphorylation of the enzyme by ATP, acetylphosphate and inorganic phosphate. 

The inner membrane itself plays an important part in oxidative phosphorylation. As it is impermeable to protons, the respiratory chain—which pumps protons from the matrix into the intermembrane space via complexes 1, 111, and IV—establishes a proton gradient across the inner membrane, in which the chemical energy released during NADH oxidation is conserved (see p. 126). ATP synthase then uses the energy stored in the gradient to form ATP from ADP and inorganic phosphate. Several of the transport systems are also dependent on the H"" gradient. 

---