Incubation stations

Incubation stations (mostly shakers, heating and cooling blocks, etc.)... 

The fluxes of phosphorus and silicon exchange between the sediment and seawater in the Bohai Sea are shown in Table 2.18 (Liu et al., 2004). Liu et al. (2004) reported the following results. In the autumn cruise, the dissolved oxygen concentration of near-bottom waters was 5.60 6.99 mg/L, saturation of 80% 99%, at our incubation stations. In the spring cruise, the dissolved oxygen concentration of near-bottom waters increased to 9.63 10.5 mg/L, saturation of 105% 112%. This implied that the exchange fluxes of nutrients in the Bohai Sea were better approached by incubation under air conditions, while the exchange fluxes of nutrients under N2 conditions should be considered as an approach of anaerobic conditions. 

Figure 5 The lead evaluation robot above is loading a 384-well microtitre plate into a liquidhandling device. The robot move up and down a track, move plates between reagent addition stations, incubators and readers...

Fig. 3. Mean concentrations of 236CYMS in mussels incubated in August 1986 at seven stations of the Kymijoki basin, Finland [32], MAT is reference station 40 km upstream of the first bleaching pulp mill in Aanekoski. Stations KUU, TOR, and KAR are 18,40, and 75 km downstream. LEH is 15 km downstream from another mill, where bleaching was stopped in 1981. PIL and HIR are stations of the Kymijoki River (outflow from the Lake Paijanne) upstream and downstream from additional large pulp milL Places of discharge are indicated as arrows...

Fig. 4. Trend of the mean contents of 236CYMS in mussels incubated at station KUU in successive years 1985-1992 [33, 35]...

Figure 5.3 Depth distribution of ammonia oxidation rate from four stations in the Eastern Tropical North Pacific. Data obtained from N-NH4 tracer incubations at simulated in situ light intensities. (FromWard and Zafiriou 1988)...

As one component of the decade-long VERTEX program, an oceanic time-series station (33°N, 139°W) was occupied for an 18-month period from October 1986 to May 1988. During this observation period, the site was visited on 7 occasions ( 90-day interval) for approximately 1 week per expedition to retrieve and redeploy a free-drifting sediment trap array, to collect water samples and to conduct experiments relevant to C- and N-cycle processes (Harrison et al., 1992 Knauer et al., 1990). The uptake and assimilation of NOa and NH4 substrates were measured during incubation experiments that were designed to assess, and correct for, isotope dilution of the added tracers. Photoautotrophic N assimilation was measured using the into protein method, described later in this section. Measurements were also made of the concentrations of NOs , NH4, DON, PON, total microbial biomass, autotrophic biomass, heterotrophic biomass, primary productivity and the export of particulate matter (Harrison et al, 1992). In many ways this was, at that time, the most comprehensive study of the marine N-cycle ever conducted in the North Pacific trades biome. 

Figure 5. Depth profiles of 6-4 PP in phage DNA, at two stations (station B and F) in the Gulf of Mexico. Filled circles PWH3a-Pl phage isolate, incubated at various water depths for an entire daily period. Open triangles natural viral communities concentrated from specific depths at the end of the solar day. [Redrawn from Weinbauer et al. 12.]...

Figure 10. Plankton incubation experiments at Rothera Station (Antarctica), 1998. January 21, 30 and February 1st clear days January 27th some clouds. Gray bars incident, biologically effective UV-B (CPD Mb-1) measured with a DNA dosimeter black bars accumulated damage in the small size fraction (0.2 to 2 fim) (CPD Mb-1) white bars accumulated damage in the large size fractions (> 10 /im). Samples were incubated from 9.00 until 19.00. Error bars represent standard deviations of the mean of at least two...

Assay steps and reactions are illustrated in Fig. 1. In Step-1, the assay starts with 100 pL of sample and incubates with 50 pL of SDB and 50 pL of T. cruzi rAg coated microparticles in the sample well of a PRISM reaction tray. After the first incubation, the tray is moved to the transfer station where the reaction mixture is flushed into the sandwich reaction well by transfer wash buffer and excessive fluid is... 

After the specimen has been applied to the slide, a distributor arm moves the slide to the proper incubator CM for the colorimetric and two-point rate enzyme tests (acid phosphatase, amylase, and lipase), PM for the potentiometric chemistries, and RT for the rate or kinetic incubator for the multiple-point rate enzyme chemistries. Temperature control within either the CM or RT incubator is maintained at 3 7 0.1 ° C by contact of the slide with the rotating thermal mass of the incubator. The products forming in the slides in either the CM or RT incubator are monitored at what are termed read stations by separate reflectance densitometers or reflectometers. There are, however, differences on how such measurements are made. For the enzyme slides in the CM incubator, at selected... 

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