In vitro platelet adhesion

A layer of adsorbed albumin reduces in vitro platelet adhesion materials that preferentially adsorb albumin will be antithrombogenic in vivo. 

In vitro platelet adhesion is related directly to in vivo thrombus formation and embolization on foreign surfaces. 

In vitro Platelet Adhesion. Figures 7 and 8 show the time course of platelet adhesion onto Silastic and PVA hydrogels when WP and PRP were used, respectively. PVA hydrogels were prepared under two different conditions by heat treatment at 150 C in the absence of glycerol and by heat treatment at 70 in the presence of glycerol by 40 wt% of PVA. The number of adhered platelets was expressed as a percentage of adhered... 

Typical SEM photographs of in vitro platelet adhesion tests (a) control PET, (b) PET-P200, (c) PET-PIK, and (d) PET-P6K. The cultivation time is 120 min. (Reproduced from Wang, J., et al.. Surf. Coat. Tech., 196,307-311,2005. With permission from Elsevier.)... 

Wu, Y., Zhou, Z., Meyerhoff, M.E. In vitro platelet adhesion on polymeric surfaces with varying fluxes of continuous nitric oxide release. J. Biomed. Mater. Res. A 81(4), 956-963 (2007)... 

In vitro platelet-tumor cell adhesion assay If the ability of tumor cells to induce platelet aggregation has a prominent role in metastasis formation, it could be expected that its inhibition by specific drugs would reduce the amount of metastases produced by tumor cells with a pronounced TCIPA activity. 

This chapter deals with a specific test of blood-surface interaction in vitro platelet retention in a column of beads (due to platelet adhesion and aggregation). Protein adsorption precedes platelet adsorption, and thus the in vitro platelet retention test involves competitive and sequential adsorption of proteins, the outcome of which produces surfaces having widely varying degrees of platelet retention. Except in the case of thrombin (3), plasma protein absorption on these surfaces has not been studied. 

PGEj is a powerful inhibitor of platelet-to-glass adhesiveness in a whole-blood system and of in vitro platelet aggregation, induced by A.D.P., noradrenaline, A.T.P., 5-H.T., thrombin and collagen. Species where aggregation inhibition has been observed include the rat, pig and man [332-337] the minimal effective concentration for activity against ADP-induced aggregation of human platelets is 0 05 pg/ml [336]. 

Dion et al. (1993) evaluated the in vitro platelet retention of the new prosthetic heart valve that was designed by FII Company and Pr. Baudet, composed of Ti6A14V titanium alloy coated with DLC (obtained by CVD). The retention and adhesion of platelets was evaluated by analyzing radioactivity on the exposed wall of test or control tubes through which a blood cell suspension containing In-labelled platelets had circulated. Their results showed that on DLC/Ti6A14V, platelets adhered twice the amount that they did on the reference material (a silicone medical-grade elastomer the behaviour of which in contact with blood is the same as that observed with the National Institutes of Health-recommended polydimethyl siloxane) (Dion et al., 1993). 

N5. Nierodjik, M. L., Plotkin, A., Kajuno, F., and Karpatkin, S., Thrombin stimulates tumor-platelet adhesion in vitro and metastasis in vivo. J. Clin. Invest. 87, 229-236 (1991). 

T. Okano, T. Aoyagi, K. Kataoka, K. Abe, Y. Sakurai, M. Shimada, I. Shinohara, Hydrophilic-hydrophobic microdomains surfaces having an ability to suppress platelet adhesion and their in vitro antithrombogenicity, J. Biomed. Mater. Res. 20 (1986) 919-927. 

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