Immunology Link

The use of immunologically linked enzyme-antibody complexes will most probably increase due to the advent of monoclonal antibodies. The preparation of such complexes is not only extremely simple (Section 11.3.2), but can be done with crude enzyme extracts. A bridging antibody (ABii k) is needed to link the enzyme-antibody complex (Ab E) to the primary antibody (Ablest) ... 

Non-competitive solid-phase EIA using immunologically linked enzyme-antibody... 

Rheumatoid factor may be the immunological link between white blood cells and the body s defense systems Generated 22 S antigen-antibody complex can activate asma kallikrein and lead to kinin formation and subsequent synovitis. ... 

Chapter 8 describes a number of generalized CA models, including reversible CA, coupled-map lattices, quantum CA, reaction-diffusion models, immunologically motivated CA models, random Boolean networks, sandpile models (in the context of self-organized criticality), structurally dynamic CA (in which the temporal evolution of the value of individual sites of a lattice are dynamically linked to an evolving lattice structure), and simple CA models of combat. 

The first application of immunologically based technology to pesticides was not reported until 1970, when Centeno and Johnson developed antibodies that selectively bound malathion. A few years later, radioimmunoassays were developed for aldrin and dieldrin and for parathion. In 1972, Engvall and Perlman introduced the use of enzymes as labels for immunoassay and launched the term enzyme-linked... 

One of the more difficult to manage aspects of compound reactivity is the potential for idiosyncratic immunological reactions to covalent protein-compound complexes. Normally the immune system does not respond to xenobiotics of molecular weight less than 1000 Daltons. When, however, a drag is covalently linked to a... 

An extract of the soluble proteins of the bacterium E. coli was provided by the Giddings lab in the Department of Microbiology and Immunology at the University of North Carolina at Chapel Hill. The details of the procedure for the preparation of this extract have been reported elsewhere (Link, 2004). 

Blotta, M.H., Marshall, J.D., De Kruyff, R.H. and Umetsu, D.T. (1996) Cross-linking of the CD40 ligand on human CD4+ T lymphocytes generates a co-stimulatory signal that upregulates IL-4 synthesis. Journal of Immunology 156, 3133-3140. 

Wakelin, D. and Donochie, A.M. (1983) Genetic control of immunity to Trichinella spiralis influence of H-2-linked genes on immunity to the intestinal phase of infection. Immunology 48, 343—350. 

Lorentz, A., Schwengberg, S., Sellge, G., Manns, M.P. and Bischoff, S.C. (2000) Human intestinal mast cells are capable of producing different cytokine profiles role of IgE receptor cross-linking and YEA. Journal of Immunology 164, 43-48. 

Mansfield, L.S. and Urban, J.F. Jr (1996) The pathogenesis of necrotic proliferative colitis in swine is linked to whipworm induced suppression of mucosal immunity to resident bacteria. Veterinary Immunology and Immunopathology 50, 1-17. 

An enzyme-linked immunosorbent assay (ELISA) has two major components. The first is the immunological reaction that occurs between an antigen and antibody. This reaction is crucial and needs careful optimization. The second compo-... 

The rapid development and sensitivity of the mass spectrometric methods can be foreseen and in the near future the labeling can be more frequently eliminated. The identification of the cross-linked peptide can be detected first with immunological methods and then the digested and cleaved fragments with specific tandem MS techniques. The different photophores hold discrete MS fingerprints, which allow fast recognition of the modified sites. 

---