Immunological detection and quantitation

Fernandez, J.A., Owen, T.G., Kurz, W.G.W. and De Luca, V. (1989) Immunological detection and quantitation of tryptophan decarboxylase in developing Catharanthus roseus seedlings. Plant Physiol, 91, 79-84. 

Gou, Y. Jaziri, M. Diallo, B. Vanhaelen-Fastre, R. Zhiri, A. Vanhaelen, M. Homes, J. Bombardelli, E. Immunological detection and quantitation of 10-deacetylbaccatin III in Taxus sp. Biol. Chem. Hoppe-Seyler 1994, 375, 281-287. 

The combination of the specificity of the antigen-antibody interaction with the exquisite sensitivity of fluorescence detection and quantitation yields one of the most widely applicable analytical tools in cell biology (1). Within the last decade, flow cytometry (FCM) has become an integral part of basic immunological research. Elaboration of this technology has been intensively stimulated by a rapidly growing sophistication in monoclonal antibody technology and vice versa (2). 

Taylor, A.V., Swanson, M.C., Jones, R.T., Vives, R., Rodriguez, J., Yunginger, J.W., Crespo, J.F. 2000. Detection and quantitation of raw fish aeroallergens from an open-air fish market. Journal of Allergy and Clinical Immunology 105(1) 166—169. 

Enzymes are used as analytical reagents for the measurement of several metabolites and substrates and in immunoassays to detect and quantitate immunological reactions. 

There have been many biological methods developed for cyanotoxins that use the bioactivity of these toxins, such as potent hepatotoxicity, neurotoxicity, cytotoxicity, enzymatic activity, and immunological interactions. All these methods, although they are useful for sensitive screening of cyanotoxins, cannot detect and quantitate individual cyanobacterial toxins. Bioassays are recommended if any of these conditions are fulfilled [5] ... 

Testing of leaked SpA is very cumbersome. SpA complexes must be disassociated prior to immunological detection. Quantitative Western blots and ELISA have been used for this purpose. Furthermore, it is very difficult to obtain antibodies which react with the antigen-binding site to SpA.146 The working range of this assay is between 0.5 and 10.0 ng/mL (coefficient of variation less than 5%), with a lower limit of detection of 0.2 ng/mL... 

The first studies dealing with the quantitative determination of CDT in sera from alcohol abusers were performed by conventional lEF with immunological detection. This method can easily detect genetic variants and has the power to resolve individual isoforms. However, it is laborious and time consuming [178,184]. Since then, a number of CDT methods of analysis, such as anion-exchange chromatography, chromatofocusing, HPLC, and CZE have been developed [176,184]. 

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