Immunogenicity studies murine monoclonal antibodies

The major drawback for plant-derived complex biopharmaceuticals is that the plant-specific xylosyl and fucosyl residues are attached to the core structure of N-gly-cans. Although Ghargelegue et al. [62] observed no immunogenic effects of a plant-derived murine monoclonal antibody in an animal study based on a mouse model, both residues are described in the Htera-ture as structures with high immunogenic potential [8, 9]. 

Because most monoclonal antibodies that have been studied for tissue targeting are from mouse or, occasionally, from rat, the problem of antibody production to such foreign proteins always exists. While murine-derived mAbs are well tolerated for acute therapy, their use in chronic therapy is limited, due to severe human anti-mouse antibody response (HAMA) [231]. The HAMA response is elicited due to the foreign nature of the antibody itself. Molecular engineering is being utilized to replace the foreign components of the murine antibody with human antibody sequences to overcome their immunogenicity [232]. 

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