Immunocytochemistry receptors

Localization of 5-HT7 receptors in rat brain by immunocytochemistry, in situ hybridization, and agonist stimulated cFos expression. J. Chem. Neuroanat. 21, 63-73. 

Understanding of the intracellular localization of steroid receptors has gone through a number of phases, beginning with the view that receptors translocated from cytoplasm to nucleus in the presence of hormone. Indeed, with the exception of thyroid hormone receptors, which are exclusively nuclear in location, cell fractionation studies have revealed that in the absence of hormone, steroid receptors are extracted in the soluble or cytosolic fraction. However, when steroid is present in the cell, many occupied receptors are retained by purified cell nuclei. Histological procedures, such as immunocytochemistry, have confirmed the largely nuclear localization of occupied receptors, but... 

Norfleet AM, Thomas ML, Gametchu B, Watson CS (1999) Estrogn receptor-a detected on the plasma membrane of aldehyde-fixed GH3/B6FIO rat pituitary tumor cells by enzyme-linked immunocytochemistry. Endocrinology 140 3805-3814... 

The hormone receptors most studied in the immunocytochemistry of tumors are the estrogen (antibodies Abbott H222-ER-ICA/ 6/ and Dako 1D5 [7]) and... 

Reiner, A., Reiner, G., Spona, J., Teleky, B., Kolb, R., and Holzner, J. H. 1987. Estrogen receptor immunocytochemistry for preoperative determination of estrogen receptor status on fine-needle aspirates of breast cancer. Am. J. Clin. Pathol. 88 399-404. 

Wagner, R., and Deleo, J. A. (1996). Pre-emptive dynorphin and N- me thyl-D- aspartate glutamate receptor antagonism alters spinal immunocytochemistry but not allodynia following complete... 

Several groups have produced and characterized anti-5-HT1A antibodies intended for immunocytochemistry, raised against synthetic peptides (14-19) or fusion proteins (20,21) corresponding to portions of the sequence of the human or rat 5-HT receptors. Until now, however, only the polyclonal antibodies produced and characterized in Michel Hamon s laboratory (14,15) have fully demonstrated their specificity and usefulness for immunocytochemistry at both the light and electron microscopic levels. 

Morilak DA, Somogyi P, Lujan-Miras R, Ciaranello RD. Neurons expressing 5-HT2 receptors in the rat brain neurochemical identification of cell types by immunocytochemistry. Neuropsychopharmacology 1994 11 157-166. 

Fig. 4. Immunocytochemistry of formalin fixed 147L) cell cultures using a-PR-A anti-receptor MAb (left) and a control MAb (right). Immunocytochemistry was performed with T47D breast cancer cells grown as monolayers in chamber slides. Cells were fixed for 15 min with 3.7% buffered formalin, followed by a permeabilization step with Triton X-100 (0.1%) for antibody penetration into the cell. Immunocytochemistry was performed by the indirect avidin-biotin immunoperoxidase method using diaminobenzi-dine as the chromagen.

Van Dijken H, Voorn P, Holstege JC (1996) Localization of dopamine D2 receptor in spinal cord identified with immunocytochemistry and in situ hybridization. Eur J Neurosci 5 621-628. 

Ultrastructural studies of the D5 dopamine receptor using immunocytochemistry have revealed that this receptor subtype is highly expressed in the human cortex in pyramidal neurons and their dendrites are present within layers IV-VI (Khan et al., 2000). The D5 dopamine receptor is also localized to the striatum, substantia nigra (both pars compacta and reticulata), the superior colliculus, the thalamus and the pyramidal cells of hippocampus (Khan et al., 2000). In the striatum, electron microscopic analysis indicates that D5 dopamine receptors are present in the spines where asymmetric synapses are formed... 

NMDA receptors have been localized by immunocytochemistry to the postsynaptic membrane of Schaffer collateral-commissural synapses (Petralia et al., 1994a,b Petralia et al., 1999 Takumi et al., 1999). 

Fig. 17. Drawings showing the relative expression levels of AMPA receptor subunit GluR-A to -D mRNAs in parvalbumin- (PV) and calretinin- (CR) immunopositive cells of the adult rat hippocampus (non-radioactive in situ hybridization combined with indirect fluorescence immunocytochemistry). Each dot represents one cell. Intensity levels are black, strongly positive grey, moderate or lightly labelled white, negative (Catania et al., 1998).

Petralia RS, Wenthold RJ (1998) Glutamate receptor antibodies. Production and immunocytochemistry. In Ariano MA (Ed), Receptor Localization Laboratory Methods and Procedures. New York Wiley-Liss, pp 46-74. 

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