Imaging lymphocytes

Inflammatory Site Imaging Lymph Node Imaging Lymphocyte Kinetics... 

A relationship between human lymphocyte antigens and nickel sensitivity exists and predicts that individuals with this antigen have a relative risk of approximately 1 in 11 of developing nickel sensitivity (Mozzanica et al. 1990). Antibodies to hydroxymethyl uracil, an oxdized DNA base, have also been shown to be increased in some nickel-exposed workers (Frenkel et al. 1994). A preliminary study using imaging cytometry of nasal smears obtained from nickel workers indicates that this method... 

Figure 31-16 Schematic depiction of lymphocyte receptors forming anti-idiotype and internal image sets as proposed by Jerne.

Fig. 6.1. Scanning electron micrographs showing the different surface textures of red (Er) and white blood cells. A Cells within a blood vessel. B,C A comparison of scanning electron micrographs with conventional light microscope images of the same field of stained cells. Enlarged pictures at the right emphasize the different surface textures of monocytes (Mo) and platelets (PI) in D, lymphocytes (Ly) in E, and neutrophils (Ne) in F. From Kessel RG and Kardon RH (1979). Tissues and Organs A Text Atlas of Scanning Electron Microscopy, WH Freeman, NY.

Figure 2. Illustration of spatial and density resolution, using a lymphocyte image as an example. (A) Fixed spatial resolution, variable density resolution 16, 4, and 2 grey levels. (B) Fixed density resolution (16 levels), variable spatial resolutions. The 16 levels are represented by , 1, 2, 3, 4, 5, 6, 7, 8, 9, A, B, C, D, E, and F.

F6. Fong, S., Gilbertson, T. A., Chen, P. P., Vaughan, J. H., and Carson, D. A., Modulation of human rheumatoid factor-specific lymphocyte responses with a cross-reactive anti-idiotype bearing the internal image of antigen. J. Immunol. 132, 1183-1189 (1984). 

Figure 16.7 Images of comets (from lymphocytes), stained with DAPI. These represent classes 0-4, as used for visual scoring. Source Collins, A.R. (2004). Molecular Biotechnology, 26, 249-261. Reprinted with permission from Humana Press Inc. Reproduced in Color plate 16.7.

The increase in phosphoinositide hydrolysis appears to occur during the G0 and early G[ phases of the cell cycle. Cells will not tolerate a sustained increase in intracellular Ca2+ and actively extrude the ion. Recent evidence in B lymphocytes, using calcium probes and image intensifies, shows that when surface immunoglobulin is crosslinked the rise of intracellular Ca2+ occurs in repeated short bursts [37]. 

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