Identification prokaryotes

Starr, M.P., Stolp, H., Triiper, H.G., Balows, A. and Schlegel, H.G. 1981 The Prokaryotes A Handbook on Habitats, Isolation, and Identification of Bacteria. Vol. 1. Berlin, Springer-Verlag. Turban-Just, S. in preparation Biogene Dekomposition von Knochenprotein. Ph.D. dissertation, Miinchen. 

Spring, S. Schulze, R. Overmann, J. Schleifer, K. H. Identification and characterization of ecologically significant prokaryotes in the sediment of freshwater lakes Molecular and cultivation studies. FEMS Microbiol. Rev. 2000, 24, 573-590. 

Wagner, M. Horn, M. Daims, H. Fluorescence in situ hybridisation for the identification and characterisation of prokaryotes. Curr. Opin. Microbiol. 2003, 6, 302-309. 

Watson SW, Valos FW, Waterbury JB. The family nitrobacteraceae. In Starr MP, Stolp H, Tmpe HG, Below AP, Shlegel HG (eds.), The Prokaryotes, A handbook on Habits, Isolation, and Identification of Bacteria. Springer-Verlag, Berlin. 1981. 

This chapter presents methods and protocols suitable for the identification and characterization of inhibitors of the prokaryotic and/or eukaryotic translational apparatus as a whole or targeting specific, underexploited targets of the bacterial protein synthetic machinery such as translation initiation and amino-acylation. Some of the methods described have been used successfully for the high-throughput screening of libraries of natural or synthetic compounds and make use of model universal mRNAs that can be translated with similar efficiency by cellfree extracts of bacterial, yeast, and HeLa cells. Other methods presented here are suitable for secondary screening tests aimed at identifying a ... 

Nielsen, H., Engelbrecht, J., Brunak, S., and von Heijne, G. (1997). Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 10, 1-6. 

Brenner DJ (1992) Introdution to the family Enterobacteriaceae. In The prokaryotes, a handbook on the biology, ecophysiology, isolation, identification, applications, vol 3, 2nd edn. Springer-Verlag, Newyork, pp 2673-2695, Chapter 141... 

Biosynthesis.—Ubiquinone. The identification of 3,4-dihydroxyhexaprenylben-zoate (162) in a Saccharomyces cerevisiae mutant strain that cannot synthesize ubiquinone suggests that (162) may be an intermediate in ubiquinone-6 biosynthesis in eukaryotes, in contrast to the pathway via 2-polyprenylphenol which operates in prokaryotes. In mammalian systems alternative routes have been discussed for ubiquinone biosynthesis in rats." Some properties of mitochondrial 4-hydroxybenzoate-polyprenol transferase have been described."" ... 

Oyama, H., Abe, S., Ushiyama, S., Takahashi, S., and Oda, K. (1999). Identification of catalytic residues of pepstatin-insensitive carboxyl proteinases from prokaryotes by site-directed mutagenesis. J. Biol. Chem., 274, 27815-27822. 

Park KS, Jang YS, Lee H, Kim JS (2005) Phenotypic alteration and target gene identification using combinatorial libraries of zinc finger proteins in prokaryotic cells. J Bacteriol 187 5496-5499... 

There are some obvious limitations to the use of prokaryotic assays for predicting responses in mammals, given the difference in cellular and genomic complexity between prokaryotes and eukaryotes. However, for hazard identification purposes, these prokaryotic assays are a useful component. 

The past few years have witnessed significant advances in establishing the gating mechanism of prokaryotic mechanosensitive channels, particularly the recent characterization of the open state of MscL (Sukharev et al., 2001a Perozo et al., 2002a) and the identification and initial... 

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