Hydrogel, micropattems

The use of PDMS was based on pressure-moulding technique of the spin-coated PEG hydrogel on asihcon substrate. The use of PDMS stamp was effective in producing PEG hydrogel micropattems of features lesser than 50-10 o,m. Photopolymerization through the PDMS stamp resulted in the fabrication of PEG hydrogel micropattems of sub-ceUular dimensions (10-40 om) and sharper resolutions. In addition,... 

Chen YM, Shen KC, Gong JP, Osada Y (2007) Selective ceU spreading, proliferation, and orientation on micropattemed gel surfaces. J Nanosci Nanotechnol 7 773 Chen YM, Tanaka M, Gong JP, Yasuda K, Yamamoto S, Shimomura M, Osada Y (2007) Platelet adhesion to human umbilical vein endothelial ceUs cultured on anionic hydrogel scaffolds. Biomaterials 28 1752-1760... 

Koh WG, Revzin A, Siminian A, Reeves T, Pishko M (2003) Control of mammalian cell and bacteria adhesion on substrates micropattemed with poly(ethylene glycol) hydrogels. Biomed Microdev 5 11-19... 

Schild HG, Muthukumar M, Tirrell DA (1991) Cononsolvency in mixed aqueous-solutions of poly (W-isopropyacrylamide). Macromolecules 24 948-952 Schmaljohann D, Nitschke M, Schulze R, Eing A, Werner C, Eichhom K-J (2005) In situ study of thermoresponsive behavior of micropattemed hydrogel films by imaging ellipsometry. Langmuir 21 2317-2322... 

Burnham MR, Turner JN, Szarowski D, Martin DL (2006) Biological functionalization and surface micropatteming of polyacrylamide hydrogels. Biomaterials 27(35) 5883-5891 Cass AGE et al (1984) Eerrocene-mediated enzyme electrode for amperometric determination of glucose. Anal Chem 56(4) 667-671... 

Sidorenko, A., et al. Reversible switching of hydrogel-actuated nanostructures into complex micropattems. Science 315(5811), 487 90 (2007)... 

Laminin, another ECM protein, has also been used to modify materials, particularly for neural applications. When laminin was covalently immobilized on 3D agarose hydrogels, neurite extension from embryonic chick DRGs was stimulated. Further, immobilization was necessary, since laminin simply mixed into the agarose gel was unable to stimulate neurite extension (Yu et al., 1999). Uniform laminin substrates have also been used for the attachment of Schwann cells, but no preferential cell orientation was shown. When micropattems of laminin and albumin were fabricated on... 

Zhu AP, Chan-Park MB, Gao JZ. Foldable micropattemed hydrogel film made from biocompatible PCL-b-PEG-b-PCL diacrylate by UV embossing. J Biomed Mater Res B Appl Biomater 2006 76(1) 76. 

In Fig. 4.7.5, a micro- star of poIyNIPAM material is shown, which has been harvested from the above-mentioned micropatteming operation. This result suggests the possibility of producing other types of two-dimensional micron-shaped polymers, including thermoreversible hydrogels. 

Recently, the simple method of replica molding has been adapted to obtain 3D microstrucrnred thermoresponsive hydrogels [59]. Replica molding is a fast, flexible and straightforward micropatteming technique that can be carried out... 

Fig. 6 Steps utilized to carry out replica molding dispensing of the polymer on the mold, crosslinking and release of the replica ELR hydrogel with micropattems such as lines or pillars, a, b ESEM micrognqrhs. a Cross section of grooves (ft = 15 mm, w = 20 mm, s = 50 mm), b Magnified surface and cross-section views of pillars (ft = 9 mm, w = s = 10 mm). Reproduced with permission [85]...

Schmaljohann, D., Nitschke, M., Schulze, R., Ring, A., Werner, C., Eichhorn, K. J. (2005). In situ study of the thermoresponsive behavior of micropattemed hydrogel films by imaging ellipsometry. Langmuir, 21, 2317-2322. 

Trkov, S., Eng, G., Di liddo, R., Pamigotto, P.P., Vunjak-Novakovic, G. Micropattemed three-dimensional hydrogel system to study human endothelial-mesenchymal stem cell interactions. J. Tissue Eng. Regen. Med. 4, 205—215 (2010)... 

Lee, S.-H., Moon, J.J., West, J.L. Three-dimensional micropatteming of bioactive hydrogels via two-photon laser scanning photolithography for guided 3D cell migration. 

---