Mediated enzyme electrodes

The CUNY Energy Institute, The City College of New York, New York, NY, USA 

Enzymatic Fuel Cells From Fundamentals to Applications, First Edition. Edited by Heather R. Luckarift, Plamen Atanassov, and Glenn R. Johnson. 

FIGURE 9.1 Concept of an electrochemical cell. The cell will have a rate-limiting process. 

---

Barton SC, Sun Y, Chandra B, White S, Hone J. 2007. Mediated enzyme electrodes which combine micro- and nanoscale supports. Electrochem Solid State Lett 10 B96-B100. 

A. Cass, G. Davis, G. Francis, H.A. Hill, W. Aston, J. Higgins, E. Plotkin, L. Scott, and A.P. Turner, Ferrocene-mediated enzyme electrode for amperometric determination of glucose. Anal. Chem. 56, 667-671 (1984). 

S. Han, M. Zhu, Z. Yuan and X. Li, A methylene blue-mediated enzyme electrode for the determination of trace mercury (II), mercury (I), methylmercury, and mercury-glutathione complex, Biosens. Bioelectr., 16 (2001) 9-16. 

Mediated enzyme electrodes were also realized on combined microscale and nanoscale supports [300]. Bioelectrocatalytic hydrogels have also been realized by co-assembling electron-conducting metallopolypeptides with bifunctional building blocks [301]. More recently, redox-modified polymers have been employed to build biofuel cells [25, 70, 302, 303]. In 2003, an enzymatic glucose/02 fuel cell which was implanted in a living plant was introduced [147]. 

A.P.F. (1984) Ferrocene-mediated enzyme electrode for amperometric determination of glucose. Analytical Chemistry, 56 (4), 667-671. 

Barton, S.C., Sun, Y.H., Chandra, B., White, S., and Hone, J. (2007) Mediated enzyme electrodes with combined micro- and nanoscale supports. Electrochemical and Solid-State Letters, 10 (5), B96-B100. 

Plotkin E V, Scott L D L and Turner A P F 1984 Ferrocene-mediated enzyme electrode for amperometric determination of glucose Anal. Chem. 56 667-1 Higgins 1 J, Hill H A O and Plotkin E V 1987 Sensor for components of a liquid mixture US Patent 4711 245... 

Burnham MR, Turner JN, Szarowski D, Martin DL (2006) Biological functionalization and surface micropatteming of polyacrylamide hydrogels. Biomaterials 27(35) 5883-5891 Cass AGE et al (1984) Eerrocene-mediated enzyme electrode for amperometric determination of glucose. Anal Chem 56(4) 667-671... 

Cass AEG, Davis G, Francis GD et al. Ferrocene-mediated enzyme electrode for the amperometric detection of glucose. Anal Chem 1984 56 667-671. 

Oyama, N., T. Ohsaka, M. Mizunuma, and M. Kobayashi (1988). Electropolymerized cobalt tetrakis(ort/w-aminophenyl) porphyrin film mediated enzyme electrode for amperometiic determination of glucose. Anal. Chem. 60(22), 2536-2537. 

Fig. 12.18 The sequence of events in a mediated enzyme electrode. and E ed are the redox forms of the enzyme Med and Medved are those of the mediator.

The complexity of a mediated enzyme electrode means that careful consideration of the system parameters is required when designing and optimizing for a particular task. One might need to take different approaches depending on the purpose of the... 

---