Biochips, cell-based

Cell-Based Biochips Fabricated Using a PMBV/PVA Hydrogel. 157... 

A mammalian cell-based biochip for allergen detection was also proposed (Matsubara et al, 2004). They cultured a basophilic mast cell line (RBL-2H3) that releases histamine upon activation by an allergen, in a microfluidic biochip molded with PDMS (poly-dimethylsiloxane). The mast cells were pretreated with a fluorescent dye, quinacrine, that binds to histamine in acidic compartments of the cell granule by mass action. Upon exposure to the allergen, the IgE-activated cells release histamine along with the fluorescent dye, which is measured by a photomultiplier tube attached to a microscope. 

N. Pereira Rodrigues, Y. Sakai and T. Fujii, Cell-based microfluidic biochip for the electrochemical real-time monitoring of glucose and oxygen, Sensors and Actuators B Chemical, 132(2), 608-613 (2008). 

Na K, Jung J, Kim O, Lee J, Lee TG, Park YH, Hyun J (2008) Smart biopolymer for a reversible stimuli-responsive platform in cell-based biochips. Langmuir 24 4917-4923... 

H.F. Cui, J.S. Ye, Y. Chen, S.C. Chong and F.S. Sheu, Microelectrode array biochip Tool for in vitro dmg screening based on the detection of a dmg effect on dopamine release from pcl2 cells, Analytical Chemistry, 78(18), 6347-6355 (2006). 

Biochip-based HIV tests have been developed by researchers at the University of Texas and Harvard Medical SchooF. The chip is placed into a toaster-sized analyser that counts the number of CD4+ cells in the drop of blood. Results are available in ten minutes and correlate well with flow cytometry (the gold standard). The device, which is being marketed by LabNow, Inc. weighs less than 2.3 kg, requires no excess reagents and is battery-operated. 

Three-Dimensional Electrodes, Fig. 3 Schematic of biochip for dielectrophoresis-based cell separation and trapping [12], (Reproduced with permission from each of the publishers)... 

Brennan LM, Widder MW, Lee LE, van der Schalie WH (2012) Long-term storage and impedance-based water toxicity testing capabilities of fluidic biochips seeded with RTgill-W1 cells. Toxicol In Vitro 26(5) 736-745. doi 10.1016/j.tiv.2012.03.010... 

Faustino, V., et al., 2014. Extensional flow-based microfluidic device deformabihty assessment of red blood cells in contact with tumor ceUs. BioChip J. 8 (1), 42-47. Available at http // link.springer.com/10.1007/S13206-014-8107-1. 

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