Human trophoblastic cells

F. Liu, M.J. Soares, and K.L. Audus. Permeability properties of monolayers of the human trophoblast cell line BeWo. Am I Physiol. 273 C1596-C1604 (1997). 

Utoguchi, N., and K.L. Audus. 2000. Carrier-mediated transport of valproic acid in BeWo cells, a human trophoblast cell line. Int J Pharm 195 115. 

Ma, T. Fiber-Based Bioreactor Systems In Mammalian Cell Culture and Tissue Engineering Human Trophoblast Cells Dissertation The Ohio State University Columbus, OH, 1999. 

Powlin, S., P.C. Keng andRJC Miller. Toxicity of cadmium in human trophoblast cells (JAr choriocarcinoma) role of calmodulin and the calmodulin inhibitor, zaldaride maleate. Toxicol. Appl. Pharmacol. 144 225 -234, 1997. 

Moreau, R., Simoneau, L.. and Lafond, J. (2001). Characteristics of calcium uptake by Be Wo cells, a human trophoblast cell line. Placenta 22,768-775,... 

A unique apical cell adhesion molecule between human trophoblastic cells and endometrial epithelial cells has been identified and designated trophinin... 

A recent study showed that trophinin not only functions as an adhesion molecule but also plays a role in signal transduction required for ErbB4 activation in human trophoblastic cells (42,43). Thus, trophinin has an activity responsible for its ability to transform silent trophectoderm cells into an aggressive trophoblast for... 

Dunk (2E, Gerllhaus A, Drewlo S, Baczyk D, Potgens AJ, Winterhagen E, Kingdom JC, Lye SJ. The molecular role of connexin 43 in human trophoblast cell fusion. Biol Reprod. 2012 86 115. doi 10.1095/bioieprod. 111.096925. 

Two species of glycoprotein are thought to be involved in the atypical cellular behaviour of human trophoblast cells. One glycoprotein is associated with localized cellular growth and contains neuraminic acid in addition to other sugars but not L-fucose, while the other contains both neuraminic acid and L-fucose and is characteristic of neoplastic cells. 

Bianchi, J. Rose, R. C. 1985. Transport of L-ascorbic acid and dehydro-L-ascorbic acid across renal cortical basolateral membrane vesicles. Biochim. Biophys. Acta 820(2) 265-273. Bode, A. M. Yavarow, C. R. 1993. Enzymatic basis for altered ascorbic acid and dehydro-ascorbic acid levels in diabetes. Biochem. Biophys. Res. Commun. 191 1347-1353. Biondi, C. Pavan, B. Dalpiaz, A. Medici, S. Lunghi, L. Vesce, F. 2007. Expression and characterization of vitamin C transporter in the human trophoblast cell line HTR-8/ SVneo Effect of steroids, flavonoids, and NSAIDs. Mol. Hum. Reprod 13(1) 77-83. Boyer, J. C. Campbell, C. E. Sigurdson, W. J. Kuo, S. M. 2005. Polarized localization of vitamin C transporters, SVCTl and SVCT2, in epithelial cells. Biochem. Biophys. Res. Commun. 334 150-156. 

Ma et al. [1997] have discussed an in vitro human placenta model for drug testing. This was a two-compartment perfusion system using human trophoblast cells attached to a chemically modified polyethylene terephthalate fibrous matrix as a cell culture scaffold. This system is a CCA in the same sense as the two-compartment system used to estimate response to dioxin. 

De Marco CS, Caniggia 1. Mechanisms of oxygen sensing in human trophoblast cells. Placenta 2002 23(suppl A) S58-S68. 

G-CSF receptors are also found on human myeloid leukaemia cells, leu-kaemic cell lines, human placenta, trophoblastic cells, vascular endothelial... 

T. Takahashi, N. Utoguchi, A. Takara, N. Yamamoto, T. Nakanishi, K. Tanaka, K.L. Audus, and Y. Watanabe. Carrier-mediated transport of folic acid in BeWo cell monolayers as a model of the human trophoblast. Placenta. 22 863-869 (2001). 

C. Wadsack, A. Hrzenjak, A. Hammer, B. Hirschmugl, S. Levak-Frank, G. Desoye, W. Sattler, and E. Malle. Trophoblast-like human choriocarcinoma cells serve as a suitable in vitro model for selective cholesteryl ester uptake from high density lipoproteins. Eur J Biochem. 270 451 -62 (2003). 

R.S. Tuan, C.J. Moore, J.W. Brittingham, J.J. Kirwin, R.E. Akins, and M. Wong. In vitro study of placental trophoblast calcium uptake using JEG-3 human choriocarcinoma cells. J Cell Sci. 98 (Pt 3) 333-342 (1991). 

H. Jin and K.L. Audus. Effect of bisphenol A on drug efflux in BeWo, a human trophoblast-like cell line. Placenta. 26 S96-S103 (2005). 

Human OCTN2 was cloned from a human placental trophoblast cell line and human kidney (77,78). OCTN2 is expressed in skeletal muscle, kidney, lung, pancreas, small intestine, adrenal gland, thyroid, and brain (77,78). OCTN2 mediates Na+-independent transport of TEA whereas... 

In humanS/ P-gp is expressed on trophoblastic cells throughout pregnancy (82/ 84-86). It has been located on apical surfaces of endodermal cells of the mouse yolk sac (87)/ in the vesicles of the brush border membrane of the human syncytiotrophoblast that directly faces maternal blood/ but not within maternal vascular endothelium (83/ 84/ 86-95). Actively transporting molecules in a basolateral-to-apical direction/ the role of P-gp within the placenta is similar to its function at other sites it extrudes drugs from the placenta back into maternal circulation/ thereby protecting the developing fetus from potential toxic factors within the maternal circulation (95). 

Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of a blastocyst stage embryo, which consists of a layer of trophoblast cells lining the ICM and blastocoel or blastocyst cavity. The ICM and trophoblast cells give rise to the embryo proper and extra-embryonic tissue, respectively. Thirthy years ago the in vitro culture of mouse ES (mES) cells was first described (Evans and Kaufman, 1981 Martin, 1981) and later in 1998 also human ES (hES) cells were derived (Thomson et al. 1998). ES cells are characterized by the unique properties of unlimited self-renewal without senescence and pluripotency. The latter infers that ES cells give rise to all cell types of the body. These specific properties led to the great scientific interest in ES cell either for their potential medical applications or as models to address more fundamental questions in development. 

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