BeWo cells

Boumah, C. E., et al. Functional expression of the nitrobenzylthioino-sine-sensitive nucleoside transporter of human choriocarcinoma (BeWo) cells in isolated oocytes of Xenopus laevis. Biochem. J. 1994, 299, 769-773. 

Huang, S. N. and P. W. Swaan. Riboflavin uptake in human tropho-blast-derived BeWo cell monolayers cellular translocation and regulatory mechanisms. J. Pharmacol. Exp. Ther. 2001, 298, 264-271. 

Rytting E and Audus KL (2005) Novel organic cation transporter 2-mediated carnitine uptake in placental choriocarcinoma (BeWo) cells. J Pharmacol Exp Ther 312(1) 192—198. 

Passive diffusion is considered to be the major pathway by which xenobiotics cross the placenta. Paracellular diffusion was shown to be the predominant pathway for transfer of hydrophilic solutes, such as chloride ions across perfused placental lobes and opioid peptides and dextrans across BeWo cells [11-13], It has been proposed that denudations in the syncytiotrophoblasts-containing fibrinoid deposits provide a possible paracellular route across the placenta [14], Transtrophoblast channels in the syncytiotrophoblasts could also be responsible for this mode of diffusion [15], For more lipophilic solutes, the transplacental route appears to be the preferred mode of passage... 

C. Ampasavate, G.A. Chandorkar, D.G. Vande Velde, J.F. Stobaugh, and K.L. Audus. Transport and metabolism of opioid peptides across BeWo cells, an in vitro model of the placental barrier. Int I Pharm. 233 85-98 (2002). 

M.L. Avery, C.E. Meek, and K.L. Audus. The presence of inducible cytochrome P450 types 1A1 and 1A2 in the BeWo cell line. Placenta. 24 45-52 (2003). 

N. Utoguchi, G.A. Chandorkar, M. Avery, and K.L. Audus. Functional expression of P-glycoprotein in primary cultures of human cytotrophoblasts and BeWo cells. Reprod Toxicol. 14 217-224 (2000). 

J.D. Aplin, A. Sattar, and A.P. Mould. Variant choriocarcinoma (BeWo) cells that differ in adhesion and migration on fibronectin display conserved patterns of integrin expression. J Cell Sci. 103 (Pt 2) 435 144 (1992). 

D.P. Cemeus and A. Van der Ende. Apical and basolateral transferrin receptors in polarized BEWO cells recycle through separate endosomes. J Cell Biol. 114 1149-1158 (1991). 

K.E. Schmid, W.S. Davidson, L. Myatt, and L.A. Woollett. Transport of cholesterol across a BeWo cell monolayer Implications for net transport of sterol from maternal to fetal circulation. J Lipid Res. 44 1909-1918 (2003). 

T. Takahashi, N. Utoguchi, A. Takara, N. Yamamoto, T. Nakanishi, K. Tanaka, K.L. Audus, and Y. Watanabe. Carrier-mediated transport of folic acid in BeWo cell monolayers as a model of the human trophoblast. Placenta. 22 863-869 (2001). 

L. Pascolo, C. Fernetti, D. Pirulli, S. Crovella, A. Amoroso, and C. Tiribelli. Effects of maturation on RNA transcription and protein expression of four MRP genes in human placenta and in BeWo cells. Biochem Biophys Res Commun. 303 259-265 (2003). 

Utoguchi, N., and K.L. Audus. 2000. Carrier-mediated transport of valproic acid in BeWo cells, a human trophoblast cell line. Int J Pharm 195 115. 

Yamamoto, T, Kuniki, K., Takekuma, Y, Hirano, T, Iseki, K., and Sugawara, M. (2007) Ribavirin uptake by cultured human choriocarcinoma (BeWo) cells and Xenopus laevis oocytes expressing recombinant plasma membrane human nucleoside transporters. European Journal of Pharmacology, 557 (1), 1-8. 

BeWo celLs appear to be an effective model for placental calcium transport (Moreau et ai, 2001) but not as good as Jar or Jeg3 cells for selective cholcsteryl ester supply to placental cells (Wadsack et ai, 2003). Tliis stresses the impuitance of comparing several models for any new. studies to find the most suitable one. 

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