Human skeletal muscle, comparison

Fagher, B Liedholm, H Sjogren, A., Monti, M. (1993). Effects of terbutaline on basal thermogenesis of human skeletal muscle and Na-K pump after 1 week of oral use—a placebo controlled comparison with propranolol. Br. J. Clin. Pharmac. 35,629-635. 

Comparison of glycosphingolipids from human and chicken skeletal muscle. The elution of gangliosides from DEAE-Sephadex A 50 column with these 0.01, 0.02 and 0.2 M sodium acetate concentrations separated the gangliosides into mono-, di- and poly-sialo- fractions. The gangliosides of human muscle are shown in Fig. 1A. The monosialogangliosides GM3, GM2 and GM1 were eluted with 0.01 M sodium acetate in methanol (lane 2), GD3 and GDla with the 0.02 M solvent (lane 4) and others with 0.2 M acetate... 

Fig. 2. (A) Analysis of human adult skeletal (HAS) and cardiac muscle (HAC) transcripts by an oligonucleotide array representing all 363 exons of the human titin gene. Left Comparison of results obtained with HAS (top) and HAC (bottom) transcripts reveals large blocks of exons that are only positive in skeletal muscle. Right Examples of constitutively expressed exons (exon 5 and 7), cardiac specific exons (11 and 49), and skeletal muscle specific exons (156 and 210). 5MM a 50mer from exon 5 including five base-pair mismatches as a control for hybridization...

Not all data confirm accumulation of protein oxidation products with age. For example, comparison of 9-month-old and 24-month-old female Long-Evans/Wistar hybrid rats did not demonstrate any age-related increase of o-tyrosine and 3-nitrotyrosine in the heart, skeletal muscle, and liver. These observations were interpreted as indications suggesting that proteins damaged by the hydroxyl radical and reactive nitrogen species did not accumulate in these tissues with advancing age (L7). Dityrosine could not be detected in human plasma proteins or haemoglobin (with the detection limit of 1 pmol/mg protein) (D3), but may accumulate in... 

Anderson PAW, Malouf NN, Oaleley AE, Pagani E, Allen PD. Troponin T isoform expression in humans a comparison among normal and failing adult heart, fetal heart and adult and fetal skeletal muscle. Circ Res 1991 69 1226-33. 

The fatty acid composition of muscle lipids may show quantitative alterations in diseased muscle. Thus lecithin isolated from human dystrophic muscle had an increased amount of oleic but diminished linoleic acid (Tl). Changes have been recorded also in the fatty acid composition of lecithin from denervated muscle (PI). Recently it has been reported (K16) that the fatty acid pattern of muscle phosphatides from patients with the autosomal dominant form of myotonia congenita differed markedly from that of the autosomal recessive form and from the normal. Tani and his co-workers (F7) have made a detailed study of the phospholipids of normal and dystrophic mouse tissues. In normal mice phosphatidylcholine and phosphatidylethanolamine from skeletal and heart muscles had a very high content of 20-22-carbon polyunsaturated acids, in comparison with those for other tissues the most abundant was docosahexaenoic acid. In dystrophic mice there was a sharp decrease in the proportion of docosahexaenoic acid in the phosphoglycerides from skeletal and heart muscles, suggesting the likelihood of important alterations in muscle membranes. Somewhat similar studies have been reported by Owens (05), who also observed a fall in the proportion of docosahexaenoic acid, mainly in the phosphatidylcholine -j- choline plasmalogen fraction. 

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