Human neutrophilic granulocytes

M. R., Ginsel, L. A. (1990). Intracellular localization and de novo synthesis of FcRIII in human neutrophil granulocytes. Blood 75,144-51. 

Shafer, W., Martin, L., Spitznagel, J. Cationic antimicrobial proteins isolated from human neutrophil granulocytes in the presence of diisopropyl fluorophosphate. Infect Immun 45 (1984) 29-35. 

Figure 9. Radioactivity profile across a SDS-polyacrylamide (5%) gel in which surface-membrane proteins of human neutrophil granulocytes have been separated. Before this experiment, isolated granulocytes had been incubated 2 h at 37°C with inIn-BLEDTA in plasma. As indicated by the arrow, a single protein with molecular weight 1.3 X 10s was...

WallerattiT, GsthL Aulitzky WE, Pollodc JS, Kleinert H, Forstermann U (1997) Identification of NO synthase isofonns expressed in human neutrophil granulocytes, megakaryocytes and platdets. Thromb Haemostas 77 163-167. 

Keller, H., Niggh, V. and Zimmermann, A. (1991). Diversity in motile responses of human neutrophil granulocytes functional meaning of cytoskeletal bias. Adv. Exp. Biol. Med. 297, 23-37. 

Phenanthrenes obtained from the Stemona species were evaluated on their leukotriene biosynthesis inhibition property using human neutrophile granulocytes in vivo. Stemanthrenes A 162 and D 165 display inhibitory activity in a dose-dependent manner, with IC50 values of 8.5 and 4.8 pM. Stemanthrenes B 163 and C 164 possess 100% inhibition against leukotriene biosynthesis at 25 pM. The phenanthrenes might be responsible for the anti-inflammatory and antiasthmatic principles of the Stemona species [154]. Denbinobin, a phenanthraquinone from Dendrobium moniliforme, shows in vitro anti-inflammatory activity. It inhibits the formation of tumor necrosis factor and prostaglandin E2 induced by lipopolysac-charide in RAW 264.7 and N9 cells at a dose of 1 pM [100]. 

Jones HA, CadwaUader KA, White JF, Uddin M, Peters AM, Chilvers ER. Dissociation between respiratory burst activity and deoxyglucose uptake in human neutrophil granulocytes imphcations for interpretation of F-FDG-PET images. J Nucl Med 2002 43 652-657. 

Aam, B. B. Fonnum, F. (+/-)-2-Chloropropionic acid elevates reactive oxygen species formation in human neutrophil granulocytes. Toxicology 2006, 228, 124-134. 

Cheng SS, Lai JJ, Lukacs NW, Kunkel SL. Granulocyte-macrophage colony stimulating factor up-regulates CCR1 in human neutrophils. J Immunol 2001 166 1178-1184. 

Lopez, A. F., Williamson, D. J., Gamble, J. R., Begley, C. G., Harlen, J. M., Klebanoff, S. J., Waltersdorph, A., Wong, G., Clark, S. C., Vadas, M. A. (1986). Recombinant human granulocyte-macrophage colony-stimulating factor stimulates in vitro mature human neutrophil and eosinophil function, surface receptor expression, and survival. 

Vadas, M. A. (1988). Recombinant human granulocyte-macrophage colony-stimulating factor (rH GM-CSF) regulates fMet-Leu-Phe receptors on human neutrophils. Immunol. 64,519-25. 

Edwards, S. W., Holden, C. S., Humphreys, J. M., Hart, C. A. (1989). Granulocyte-macrophage colony-stimulating factor (GM-CSF) primes the respiratory burst and stimulates protein biosynthesis in human neutrophils. FEBS Lett. 256,62-6. 

Edwards, S. W., Watson, F., MacLeod, R., Davies, J. M. (1990). Receptor expression and oxidase activity in human neutrophils Regulation by granulocyte-macrophage colony-stimulating factor and dependence upon protein biosynthesis. Biosci. Rep. 10, 393-401. 

McColl, S. R., Paquin, R., Menard, C., Beaulieu, A. D. (1992). Human neutrophils produce high levels of the interleukin 1 receptor antagonist in response to granulocyte/ macrophage colony-stimulating factor and tumor necrosis factor a. J. Exp. Med. 176, 593-8. 

S., Takaku, F. (1990). Granulocyte colony-stimulating factor stimulates human mature neutrophilic granulocytes to produce interferon-O . Blood 75, 17-19. 

This ligand has a lower affinity for adenosine A, and A2B receptors with pK values <6. A potent inhibitory effects on the generation of reactive oxygen species from human neutrophils and eosinophils and on the degranulation of human granulocytes subsequent to treatment with compound 30 have been described. This findings provide a useful tool for the understanding of the involvement of A2a and A3 ARs in inflammation processes. 

Takemura et al. [155] described TUNEL positivity, and Hayakawa et al. [152] described caspase-3 positivity in infiltrated and proliferated interstitial cells in an experimental model of MI, suggesting that apoptosis might play an important role in the disappearance of these cells after MI. Consistent with these studies, we found positive reaction for cleaved caspase-3 in neutrophil granulocytes in the interstitium in acute MI in humans (Fig. 4B). 

Kapp, A., Zeck Kapp, G., Danner, M., and Luger, T. A. (1988) Human granulocyte-macrophage colony stimulating factor An effective direct activator of human polymorphonuclear neutrophilic granulocytes. J. Invest. Dermatol. 91,49-55. 

Melani, C., Mattia, G.F., Silvani, A., Care, A., Rivoltini, L., Parmiani, G. and Colombo, M.P. (1993). Interleukin-6 expression in human neutrophil and eosinophil peripheral blood granulocytes. Blood 81, 2744-2749. 

Stankova J, Rola-Pleszczynski M, Dubois CM (1995) Granulocyte-macrophage colony-stimulating factor increases 5-lipoxygenase gene transcription and protein expression in human neutrophils. Blood 85 3719-3726... 

In humans, immunotoxic effects have been clearly seen among the victims of the poisoning in Turkey and also in workers exposed occupationally to hexachlorobenzene in a chemical plant in Brazil. In these workers, impaired functions of neutrophilic granulocytes and increased serum IgM and IgG levels were observed (Queiroz et ah, 1998a, 1998b). 

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