Human immunodeficiency virus type probing

Assay for human immunodeficiency virus type 1 (HIV-1) proviral DNA in peripheral blood monuclear cells can be performed by PCR followed by detection of PCR products by electrochemiluminescence-labeled oligonucleotide probe [Tris-bipyridine ruthenium (II) complex]. Since one of the PCR primers is biotin-labeled at the 5 end, facile capture of the PCR product-probe complex can be accomplished on streptavidin-conjugated magnetic particles, prior to analysis in an electrochemiluminescence analyzer (S3). 

Schutzbank, T. E., and Smith, J., Detection of human immunodeficiency virus type I proviral DNA by PCR using an electrochemiluminescence tagged probe. J. Clin. Microbiol. 33, 2036-2041 (1995). 

Stuyver, L., Wyseur, A., Rombout, A., Louwagie, J., Scarcez, T Verhofstede C., Rimland, D., Schinazi, R. F., and Rossau, R. (1997) Line Probe Assay for rapid detection of drug-selected mutations in the human immunodeficiency virus type 1 reverse transcriptase gene. Antimicrobiol. Agents. Chem. 41, 284-291. 

Fu, H., Guthrie, J. W., Le, X. C. (2006). Study of binding stoichiometries of the human immunodeficiency virus type 1 reverse transcriptase by capillary electrophoresis and laser-induced fluorescence polarization using aptamers as probes. Electrophoresis 27, 433-441. 

Choi, W.-T., Tian, S., Dong, C.-Z., Kumar, S., Liu, D., Madani, N., et al. (2005). Unique hgand binding sites on CXCR4 probed by a chemical biology approach Implications for the design of selective human immunodeficiency virus type 1 inhibitors. Journal of Virology, 79(24), 15398-15404. Retrieved from, http / www.hubmed.org/display.cgi uids=16306611. 

The detection of HIV-related proteins is one of the most challenging tasks. This is especially true because AIDS should be diagnosed as early as possible to enable an early and effective therapy of this infection. Pavski and Le (57) used the aptamer strategy to detect reverse transcriptase (RT) of the type 1 human immunodeficiency virus (HIV-1). A direct and specific ACE method was proposed using laser-induced fluorescence (ACE/LIF) as detection principle. Single-stranded DNA aptamers as probes fluorescently labeled were synthesized. The resulting aptamer is specific for HIV-1 RT, and it exhibited no cross-reactivity with RTs of the enhanced avian myeloblastosis virus (AMV), the Moloney murine leukemia virus (MMLV), or denatured HIV-1 RT. An affinity complex of RT 26-HIV-l RT was stable, with calibration curves linear up to 50 nM (6 /xg/mL) HIV-1 RT concentration. Both... 

Electrocatalytic oxidation of guanosine residues by electrogenerated tris(2,2 -bipyridyl)ruthenium(III) has recently been shown to provide a useful transduction mechanism in DNA biosensors [212). Probe strands in which inosine was substituted for guanosine were immobilized on an electrode surface, and these hybridized selectively with targets that contained oxidizable guanosine residues PCR-amplified genomic DNA from herpes simplex virus type II, Clostridium perfrin-gens, and human immunodeficiency virus were detected by this method. 

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