Line-probe assay

Line Probe Assay for Detecting Mutations in HIV-1 Reverse Transcriptase... 

Stuyver, L., Wyseur, A., Rombout, A., Louwagie, J., Scarcez, T Verhofstede C., Rimland, D., Schinazi, R. F., and Rossau, R. (1997) Line Probe Assay for rapid detection of drug-selected mutations in the human immunodeficiency virus type 1 reverse transcriptase gene. Antimicrobiol. Agents. Chem. 41, 284-291. 

Results of a dot-blot or line-probe assay are usually qualitative if hybridization has occurred, a signal is generated at the specified spot and a simple yes/no interpretation is given. As the number of probes or samples increases, it becomes challenging to find a hybridization condition that provides high stringency for all probe-target combinations. 

Figure 37-20 Two modes of dot-blot and line-probe assays. In the sampie-down mode, DNA, cDNA, RNA, or amplified products are attached to the solid support and hybridized to labeled probes in solution. Alternatively, different probes are spotted onto the support ( probe-down ) and the sample is in solution.

Andonov A, Chaudhary R. Subtyping of hepatitis C virus isolates by a line probe assay using hybridization. J Clin Microbiol 1995 33 254-6. 

Stuyver L, Rossau R, Wyseur A, Duhamel M, Vander-borght B, Van Heuverswyn H, et al. Typing of hepatitis C virus isolates and characterization of new subtypes using a line probe assay. J Gen Virol 1993 74 1093-102. 

Stuyver L, Wyseur A, Van Arnhem W, Hernandez F, Maertens G. Second-generation line probe assay for hepatitis C virus geriotyping. J Clin Microbiol 1996 34 2259-66. 

Mitchell P, Sloan L, Majewski D, Rys P, Heimgartner P, Rosenblatt J, et al. Comparison of line probe assay and DNA sequencing of 5 untranslated region for genotyping hepatitis C virus Description of novel line probe patterns. Diagn Microbiol Infect Dis 2002 42 175-9. 

Lopes J, Pacheco T, Pessanha M, Quirim J, Sancho L, Salfinger M, Amaral L. Direct application of the INNO-LiPA Rif.TB line-probe assay for rapid identification of Mycobacterium tuberculosis complex strains and detection of rifampin resistance in 360 smear-positive respiratory specimens from an area of high incidence of multidrug-resistant tuberculosis. J Clin Microbiol 2005 43(9) 4880-4. 

Sekiguchi J, Nakamura T, Miyoshi-Akiyama T, Kirikae F, Kobayashi I, Augus-tynowicz-Kopec E, Zwolska Z, Morita K, Suetake T, Yoshida H, Kato S, Mori T, Kirikae T. Development and evaluation of a line probe assay for rapid identification of pncA mutations in pyrazinamide-resis-tant Mycobacterium tuberculosis strains. J Clin Microbiol 2007 45(9) 2802-7. 

Giannoni F, Iona E, Sementilli F, Brunori L, Pardini M, Migliori GB, Orefici G, Fattorini L. Evaluation of a new line probe assay for rapid identification of gyrA mutations in Mycobacterium tuberculosis. Antimicrob Agents Chemother 2005 49(7) 2928-33. 

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