Human fetal osteoblast cells

Fulvestrant has been reported to decrease both insulin-like growth factor I (IGF-I) stimulated cell growth and IGF-I receptor mRNA (Huynh et al. 1996). Moreover, in the human fetal osteoblast cell line (hFOB/ER9 cells), both ICI 164384 and fulvestrant blocked the estradiol-induced increase in IGF-I mRNA levels (Kassem et al. 1998). 

Ozkan S, Kalyon DM, Yu X. Functionally graded beta-TCP/PCL nanocomposite scaffolds in vitro evaluation with human fetal osteoblast cells for bone tissue engineering. J Biomed Mater Res A 2010 92(3) 1007-18. 

Cell seeding. Human fetal osteoblasts (hFOB 1.19, CRL-11372, American Type Culture Collection) were seeded at an initial density of 1 x 104 cells cm 2 in a medium containing a 1 1 mixture of Dulbecco s Modified Eagle s Medium (DMEM) without phenol red and Ham s F12 medium and supplemented with 0.3 mg/ml G418, antibiotics (100 U/ml penicillin and 100 pg/ml streptomycin) and 10% foetal bovine serum. Cells were grown for 48 h at 37°C in an humidified atmosphere of 5% C02 on the cell culture plastic supports (control), and sucrose thin films deposited on glass substrates (SI substrate). Prior to cell culture, the samples were sterilized by UV light exposure. 

One of the purposes of this study was to investigate the effects of sucrose thin films deposited on glass substrates on cell morphology and viability, in an in vitro human fetal osteoblast system. Comparative morphological examination of the cell monolayer adhered to the plastic support (control) and sucrose thin films (S1), by phase contrast microscopy, showed no differences in cell shape and density. 

Verdanova, M., Pytlik, R., Kalbacova, M.H., 2014. Evaluation of sericin as a fetal bovine serumreplacing cryoprotectant during freezing of human mesench3mal stromal cells and human osteoblast-like cells. Biopreservation and Biobanking 12 (2), 99—105. 

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