HPLC determination of carotenoid pigments in human and animal tissues

2 HPLC determination of carotenoid pigments in human and animal tissues 

As it has been suggested that epoxy-j0-carotenes may reduce the incidence of lung cancer the intestinal absorption of 5,6-epoxy-/1-carotene and 5,8-epoxy-/)-carotene in humans after oral administration has been investigated by RP-HPLC. Capillary blood samples 

QUANTITIES OF CROCIN 1 1 AND CROCETIN IN HERBS OBTAINED FROM DIFFERENT 

A C30 RP column has been applied for the separation of carotenoids present in various matrices such as human serum, raw and thermally processed carrots, a Duniella algae-derived preparation and a poultry feed. Samples were mixed with deionized water and CaC03, homogenized and extracted with acetone-hexane (1 1, v/v). 

Extracts were further purified on neutral alumina cartridges conditioned by passing through 5 ml of hexane. Extracts were loaded in hexane and washed by 5 ml of hexane. The at- and /1-carotenes were removed by 3.5 ml of acetone-hexane (10 90, v/v), other carotenoids were eluted with acetone-hexane 30 70 and 70 30 v/v. Prepurification of pigments was performed in subdued light under a stream of nitrogen. Analyses were carried out in a C30 column (250 X 4.6 mm i.d., particle size 5/tm) using isocratic mobile phase composed of methyl-ferf-butyl ether (MTBE)-methanol (3 97 and 38 62, v/v) at a flow rate of 1 ml/min. The column was not thermostated separations were achieved at room temperature (about 23°C). Carotenoids were detected at 453 and 460 nm (lutein). The 

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