High Performance Liquid Chromatography - Infrared Spectroscopy

10 High Performance Liquid Chromatography - Infrared Spectroscopy [Pg.158]

In the work discussed so far in this chapter, IR spectroscopy (or UV spectroscopy) has been merely used by scanning the column effluent at a simple appropriate wavelength in order to detect particular compounds as they are eluted in the column effluent. [Pg.158]

Dwyer [51] used a combination of chromatography and IR spectroscopy to provide a versatile tool for characterisation of polymers. HPLC-Fourier-transform IR spectroscopy interface systems deposit the output of a chromatograph on an IR optical medium, which is then scanned to provide data as a time-ordered set of spectra of the chromatogram. Polymer analysis applications described include the identification of polymer additives, the determination of composition/molecular weight distributions in copolymers, the mapping of components of polymer alloys and blends, molecular configuration changes in polymers, and component identification in complex systems. [Pg.158]

Jansen [52] developed an on-line LC-IR system for the analysis of additives in polymer extracts. [Pg.158]

Ultraviolet spectra of individual pure components of mixture [Pg.159]

High performance liquid chromatography, infrared spectroscopy, UV and visible spectrophotometry, and polarography are some of the other major analytical techniques used to determine many diverse classes of compounds. [Pg.104]

High Performance Liquid Chromatography-Infrared Spectroscopy... [Pg.87]

Other combinations of chromatography techniques with MS which may be useful in environmental studies are the coupling of high performance liquid chromatography (LC) with MS [84,384,504,506,530,585-593],LC with MS-MS [181, 594 - 599], LC with atmospheric pressure chemical ionization MS (LC-APCI-MS) [600], and Fourier transform infrared spectroscopy-fast atom bombardment coupled to LC-MS (FTIR-FAB-LC-MS) [514]. [Pg.79]

Schulten, H.-R., and Soldati, F. (1981). Identification of ginsenosides from Panax ginseng in fractions obtained by high-performance liquid chromatography by field desorption mass spectrometry, multiple internal reflection infrared spectroscopy and thin layer chromatography. J. Chromatogr. 212, 37-i9. [Pg.93]

Huck, C. et al., Quantitative Fourier transform near infrared reflectance spectroscopy (NIRS) compared to high performance liquid chromatography (HPLC) of a flavone in Primulae veris Flos extracts, Pharm. Pharmacol Lett., 9, 26, 1999. [Pg.137]

INFRARED TECHNOLOGY AND RAMAN SPECTROSCOPY - INFRARED TECHNOLOGY] (Vol 14) Hplc. See High performance liquid chromatography. [Pg.484]

We will first describe briefly the main experimental techniques coupled with electrochemical methods Infrared Reflectance Spectroscopy (IRS), Electrochemical Quartz Crystal Microbalance (EQCM), Differential Electrochemical Mass Spectrometry (DEMS), Chemical Radiotracers and High Performance Liquid Chromatography (HPLC). [Pg.399]

There are a variety of analytical methods commonly used for the characterization of neat soap and bar soaps. Many of these methods have been published as official methods by the American Oil Chemists Society (29). Additionally, many analysts choose United States Pharmacopoeia (USP), British Pharmacopoeia (BP), or Pood Chemical Codex (FCC) methods. These methods tend to be colorimetric, potentiometric, or titrametric procedures. However, a variety of instmmental techniques are also frequendy utilized, eg, gas chromatography, high performance liquid chromatography, nuclear magnetic resonance spectroscopy, infrared spectroscopy, and mass spectrometry. [Pg.159]

In order to investigate the properties of individual fractions of humic substances, various modes of high performance liquid chromatography (HPLC) have been employed. Hydrophobic interaction chromatography (5) has proved to be an effective separation technique, resulting in five distinct humic fractions from one sample. Structural analysis of these fractions was subsequently performed by infrared and nuclear magnetic resonance spectroscopy, and molecular weight distribution was also measured. [Pg.141]

DiNunzio, J. E. Pharmaceutical applications of high-performance liquid chromatography interfaced with Fourier transform infrared spectroscopy. Journal of Chromatography. 626 97-107, 1992. [Pg.358]