High-performance liquid atomic fluorescence spectrometry

E. Puskel, Z. Mester, P. Fodor, Determination of selenoamino acids by high-performance liquid chromatography-hydraulic high pressure nebulization-atomic fluorescence spectrometry, J. Anal. Atom. Spectrom., 14 (1999), 973-976. 

E. Ramalhosa, S. Rio Segade, E. Pereira, C. Vale and A. Duarte, Simple methodology for methylmercury and inorganic mercury determinations by high-performance liquid chromatography-cold vapour atomic fluorescence spectrometry. Anal. Chim. Acta, 448(1-2), 2001, 135-143. 

Notes LOD, limit of detection MeOH, methanol EtOH, ethanol ACN, acetonitrile MTBE, methyl tert-butyl ether DCM, dichloromethane THF, tetrahy-drofuran KOH, potassium hydroxide SFE, supercritical fluid extraction MS, mass spectrometry HPLC, high-performance liquid chromatography DAD, diode array detector PDA, photodiode array detector FD, fluorescence detector ECD, electrochemical detector ESI, electrospray ionization APCI, atmosphere pressure chemical ionization TLC, thin layer chromatography FAB, fast atom bombardment NMR, nuclear magnetic resonance BHT, butylated hydroxytoluene SPE, solid phase extraction. 

Figure 6.1 Bar-graph of MeHg in CRM 580. The results correspond to six replicate determinations as performed by different laboratories using various methods. MEANS indicates the mean of laboratory means with 95% confidence interval. Abbreviations-. CVAAS, cold vapour atomic absorption spectrometry CVAFS, cold vapour atomic fluorescence spectrometry ECD, electron capture detection GC, gas chromatography HPLC, high-performance liquid chromatography ICPMS, inductively coupled plasma mass spectrometry MIP, microwave induced plasma atomic emission spectrometry QFAAS, quartz furnace atomic absorption spectrometry SFE, supercritical fluid extraction.

L. N. Liang, G. B. Jiang, J. G. Liu, J. T. Hu, Speciation analysis of mercury in seafood by using high-performance liquid chromatography on-line coupled with cold-vapor atomic fluorescence spectrometry via a post column microwave digestion, Anal. Chim. Acta, Ml (2003), 131-137. 

AED = atomic emission detection FI = fluorescence FID = flame ionization detection GC = gas chromatography FIMW= high molecular weight FIPLC = high-performance liquid chromatography LMW= low molecular weight MS = mass spectrometry PAFI = polycyclic aromatic hydrocarbon UV = ultraviolet... 

More recently for ultratrace determination and speciation of antimony compounds the so-called hyphenated instrumental techniques have been applied which combine adequate separation devices with suitable element-specific detectors. They include high-performance liquid chromatography (HPLC) connected on-line with heated graphite furnace (HGF) AAS (HPLC-HGF-AAS), hydride-generation atomic fluorescence spectrometry (HPLC-HG-AFS) or inductively coupled plasma (ICP) mass spectrometry (MS) (HPLC-ICP-MS) capillary electrophoresis (CE) connected to inductively coupled plasma mass spectrometry (CE-ICP-MS) and gas chromatography (GC) coupled with the same detectors as with HPLC. Reliable speciation of antimony compounds is still hampered by such problems as extractability of the element, preservation of its species information, and availability of Sb standard compounds (Nash et al. 2000, Krachler etal. 2001). Variants of anodic stripping voltammetry for speciation of antimony have also been applied (Quentel and Eilella 2002). 

High Performance Liquid Chromatography Electrophoresis Thin Layer Chromatography Ultraviolet and Visible Spectroscopy Fluorescence and Phosphorescence Atomic Absorption and Plasma Spectroscopy X-ray Methods Mass Spectrometry... 

A Woller, Z Mester, P Fodor. Determination of arsenic species by high-performance liquid chromatography-ultrasonic nebulization atomic fluorescence spectrometry. J Anal At Spectrom 10 609-613, 1997. 

ISE, ion-selective electrode FAAS, flame atomic absorption spectrometry ETAAS, electrothermal atomic absorption spectrometry FES, flame emission spectrometry FIG, hydride generation CV, cold vapor AFS, atomic fluorescence spectrometry Bl, biamperometry FIPLC, high-performance liquid chromatography LC, liquid chromatography GC-MS, gas chromatography-mass spectrometry Br-PADAP, 2- 5-bromo-2-pyridylazo)-5-(diethylamino)phenol Cig, octadecyl-chemically modified silicagel PAN, 1-(2-thiazolylazo)-2-naphthol. 

Figure 1 Steps for determination of organomercury compounds. CV-AAS, cold vapor-atomic absorption spectrometry CV-AFS, cold vapor-atomic fluorescence spectrometry GC, gas chromatography GC-ECD, gas chromatography-electron capture detection AED, atomic emission detector ICP-MS, inductively coupled plasma-mass spectrometry HPLC, high-performance liquid chromatography.

Methods also employed for determination of inorganic lead include isotope dilution mass spectrometry, flame atomic fluorescence spectrometry, and molecular absorption spectrometry, but these methods are not used for routine applications. Methods for the determination of organolead speciation are high-performance liquid chromatography with a separation of the species and flame AAS or UV detection. 

Simplex methodology to measure methylmercury and inorganic Hg by high-performance liquid chromatography-cold vapour atomic fluorescence spectrometry. 

C. Moscoso-Perez, J. Moreda-Pineiro, P. Lopez-Mahia, S. Muniategui-Lorenzo, E. Fernandez-Fernandez and D. Prada-Rodriguez, Pressurized liquid extraction followed by high performance liquid chromatography coupled to hydride generation atomic fluorescence spectrometry for arsenic and selenium speciation in atmospheric particulate matter, J. Chromatogr. A, 2008, 1215(1-2), 15-20. 

Potin-Gautier M, Pannier F, Quiroz W, Pinochet H, de Gregori I (2005) Antimony specia-tion analysis in sediment reference materials using high-performance liquid chromatography coupled to hydride generation atomic fluorescence spectrometry. Anal Chim Acta... 

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