High performance capillary electrophoresis applications

Vol. 146. High Performance Capillary Electrophoresis Theory, Techniques, and Applications. Edited by Morteza G. Khaledi... 

Macrocyclic Compounds in Analytical Chemistry. Edited by Yury A. Zolotov Surface-Launched Acoustic Wave Sensors Chemical Sensing and Thin-Film Characterization. By Michael Thompson and David Stone Modern Isotope Ratio Mass Spectrometry. Edited by T. J. Platzner High Performance Capillary Electrophoresis Theory, Techniques, and Applications. Edited by Morteza G. Khaledi... 

MG Khaledi, ed. High-Performance Capillary Electrophoresis. Theory, Techniques and Applications. New York Wiley, 1998. 

Factors Affecting Ionic Migration. Effect of Temperature. pH and Ionic Strength. Electro-osmosis. Supporting Medium. Detection of Separated Components. Applications of Traditional Zone Electrophoresis. High-performance Capillary Electrophoresis. Capillary Electrochromatography. Applications of Capillary El ectrochromatography. ... 

Shibukawa et al. (40) discussed the frontal analysis method, also called high-performance frontal analysis (HPFA) or high-performance capillary electrophoresis/frontal analysis (HPCE/FA), compared it to conventional methods, and focused on the application to stereoselective protein binding. The affinity of the drugs warfarin, verapamil, and carbamazepine and the drug candidate BOF-4272 to HSA was investigated. 

CA Academic Press, 2000) T. Wehr, R. Rodrfguez-Diaz, and M. Zhu, Capillary Electrophoresis of Proteins (New York Marcel Dekker, 1999) M. G. Khaledi, ed., High Performance Capillary Electrophoresis Theory, Techniques, and Applications (New York Wiley, 1998) P. Camilleri, ed., Capillary Electrophoresis Theory and Practice (Boca Raton, FL CRC Press, 1998) J. P. Landers, Handbook of Capillary Electrophoresis, 2nd ed. (Boca Raton, FL CRC Press, 1997) H. Shintani and... 

The reader is directed to Ref. 5, which makes an interesting comparison between HPLC and other analytical methodologies for the determination of carbohydrates in foods. Additionally, notable progress has been made in the application of high-performance capillary electrophoresis (HPCE) in this field (8-11). However, given the scope of this chapter, we will focus on the advantages and drawbacks of other chromatographic techniques versus HPLC. 

High performance capillary electrophoresis is one form of free-solution electrophoresis. CE is useful to researchers and analysts working in areas in which traditional electrophoresis is customarily applied (e.g., biopolymer analysis) and also in disciplines not usually associated with electrophoretic analysis, such as inorganic ion analysis. The potential application areas of CE are vast, because this technique can separate a variety of ligates, from inorganic ions up to intact cells, using the same instrumental hardware designed for separations based on different physical-chemical mechanisms. 

In this book, the focus is on the use of high performance liquid chromatography (HPLC) for the separation and detection of biochemical components produced from enzymatic reactions. But the purpose of the book is also to introduce HPLC and high performance capillary electrophoresis (HPCE) as methods applicable for separation and detection of a variety of components, even those not produced by enzymatic reactions. 

Kajiwara H (1991) Application of high-performance capillary electrophoresis to the analysis of conformation and interaction of metal-binding proteins. J Chromatogr 559 345-356. 

Kenndler, E., J. Microcol. Separ. 10 273 (1998). Kenndler, E., in High Performance Capillary Electrophoresis, Theory, Techniques and Applications, M. G. Khaledi (ed.), John Whey Sons, New York, 1998, Vol. 146, pp. 25-76. 

Electrophoretic assays may be used to characterize the purity and homogeneity of biopharmaceuticals, based mainly on an ability to differentiate chemical and molecular changes in the compound as a result of oxidation, denaturation, aggregation, and deamidation [11]. Well-established techniques include isoelectric focusing (lEF) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), whilst high-performance capillary electrophoresis (HPCE), utilizing a variety of detection systems, may also be used for routine applications. 

D Holme and H. Peck, Analytical Biochemistry, 3rd ed. (1998), Addison Wesley Longman (Essex), pp 132-146 Introduction to all techniques of electrophoresis M Khaledi, High Performance Capillary Electrophoresis Theory, Techniques, and Applications (1998), John Wiley Sons (New York) Up-to-date coverage of CE W. Ruhr, Anal Chem. 62, 403R-414R (1990) Capillary Electrophoresis ... 

Khaledi, M. G, High-performance capillary electrophoresis theory, techniques, and applications, in Chemical Analysis A Series of Monographs on Analytical Chemistry and its Applications, 1st ed., Winefordner, J. D. (ed.), Wiley-Interscience, Raleigh, 1998, pp. 308. 

---