Hemoglobin expressing concentration

Hb = measured hemoglobin expressed as a percentage of the normal hemoglobin concentration where 14.8% hemoglobin is considered as 100% concentration. If the patient weighs 30 lbs or less, the dose should be 80% of the calculated amount. 

Hemoglobin (Hb) behaves differently. Its sigmoidal binding curve can be fitted by an association-constant expression with a greater-than-first-power dependence on the oxygen concentration ... 

The sorption efficiency of MC was determined as the ratio of the quantity of the adsorbed substance to its initial amount (w / w), expressed in % for a certain ratio (w / w) of adsorbent to substance. Optimal ratios of adsorbent to substance equal 15-20 for barbiturates, 20 - 25 for cyanocobalamin and bilirubin, and 40 - 50 for hemoglobin. The initial concentration of absorbed substances was 100 - 200 pg/ml. The substances were incubated for lmin with MC either in physiological solution or in donor plasma and donor blood at room temperature (pH 7.4). The concentration of substances in the solutions was measured by differential visual and UV-spectroscopy. Concentrations of substances in blood and plasma and adsorption of total plasma proteins was determined by thin-layer chromatography with a fluorescent label. 

The fundamental quantity to be used in any standardized spectro-photometric determination of hemoglobin should be the quarter millimolar extinction coefficient because of the four Fe atoms per hemoglobin molecule. This quantity is independent of the molecular weight of hemoglobin. Consequently, a redetermination of the molecular weight will not change the value of the extinction coefficient to be used and will not affect hemoglobin concentrations when these are expressed in quarter millimoles per liter. 

The erythrocyte precursor cell types display a continuum of changes in shape, hemoglobin concentration, Rh antigen, and erythropoietin (EPO) receptor expression with maturity. However, mature erythrocytes express significantly lower EPO receptor density than do proerythroblasts. ... 

While this concept for the control of erythropoiesis is generally accepted, there are at present insufficient data to determine quantitative relationships among the variables involved. In addition, some auxiliary systems must be included to describe fully the erythropoietic response to stress such as hypoxia. The performance of the respiratory system and the relationships expressed by the oxyhemoglobin dissociation curve affect the oxygenation state of the blood. Blood volume, and in particular plasma volume, affect hemoglobin concentration which limits the amount... 

Other measurements used to classify the type of anemia present include the mean corpuscular volume (MCV) and the mean corpuscular hemoglobin concentration (MCHC). The MCV is the average volume of the red blood cell, expressed in femto (10 ) liters. Normal MCV range from 80 to 100 fL. The MCHC is the average concentration of hemoglobin in each individual erythrocyte, expressed in g/L. The normal range is 32 to 37 a value of less than 32 would indicate hypochromic cells. Thus, microcytic, hypochromic red blood cells have an MCV of less than 80 and an MCHC of less than 32. Macrocytic, normochromic cells have an MCV of greater than 100, with an MCHC between 32 and 37. 

Concentrations, expressed per heme, were determined by the Drabkin method and were all close to 18 mM. Concentrations of.CO--heme were also determined breaking the cells in distilled water and using known extinction coefficient of CO-hemoglobin results of both methods were invariably found to be equal in the experimental error. Magnetic volume susceptibility measurements were performed with our superconducting susceptometer (25) as described elsewhere (23). The overall accuracy of the method for protein aqueous solutions referred to calibrations with pure deoxygenated water is 7x10" cgs units, or 1/1000 of the volume susceptibility of water. 

High sensitivity to low oxygen concentrations. Many microbial cells are sensitive to low oxygen concentration, which may result in reduced product formation—or even irreversible loss of product formation—or onset of fermentative metabolism. This problem can partly be overcome by expression bacterial hemoglobin, which has demonstrated to have a positive influence in many different fermentation processes. 

---