Hemoglobin derivatives

Blood Hemoglobin derivatives (HbCO, MetHb) Spectrophotometric Derivatization GC... 

Szebeni J, Hauser H, Eskelson CD, et al. Interaction of hemoglobin derivatives with liposomes. Membrane cholesterol protects against the changes of hemoglobin. Biochemistry 1988 27 6425. 

With reference to the octahedral energy level diagrams for oxy, car-boxy and deoxy hemoglobin derived from extended Huckel calculations and our earlier work on the natural heme complexes, the results of this study can be discussed. 

FIGURE 5-6 A comparison of the structures of myoglobin (PDB ID 1MBO) and the j3 subunit of hemoglobin (derived from PDB ID 1 HCA). 

The use of picosecond pulses to minimize the Interference of fluorescence with the Raman spectrum was also demonstrated (5) at about that time. The use of vldicon detection in Raman spectroscopy was demonstrated (6) in 1976. The first resonance Raman spectrum taken for a photobiologlcal system (bacteriorhodopsin) in the nanosecond time scale was (7) in 1977. The resonance Raman spectra of bacteriorhodopsin have also been measured in the microsecond (8,9,10) and in the millisecond (11) time domain. Recently the time resolved resonance Raman spectra of photolyzed hemoglobin derivatives have been reported (12). 

A polyethylene glycol-conjugated hemoglobin derivative and carbon monoxide carrier, CO-MP4, is able to deliver CO to the circulation and reduces ischemia/ reperfusion injury in rats, providing the first evidence for this drug as a CO therapeutic agent [191, 192]. 

Tesoriere L, Allegra M, D Arpa D et al (2001) Reaction of melatonin with hemoglobin-derived oxoferryl radicals and inhibition of the hydroperoxide-induced hemoglobin denatur-ation in red blood cells. J Pineal Res 31 114-119... 

The question has been essentially decided by more recent experiments (Beychok and Steinhardt, 1959 Steinhardt et al., 1962). One procedure was to reduce the temperature and increase the ionic strength, the increase in ionic strength being for the purpose of diminishing the importance of electrostatic interactions. A second procedure was to use hemoglobin derivatives which are especially stable in the native state, these being the... 

Given the hurdles and the costs associated with the development of a clinical product that is a new biologic, it is remarkable that at least five companies continue to be engaged in clinical trials of hemoglobin-based oxygen carriers (Table 2). Most of the companies are developing modified hemoglobins derived from natural sources (human or bovine red... 

Kumar, R. Manjula, B.N. Human hemoglobin derived from transgenic swine a starting material for blood substitute formulations. In Red Blood Cell Substitutes Rudolph, A.S., Rabinovici, R., Feuerstein, G.Z., Eds. Marcel Dekker, Inc. New York, 1998 309-324. 

Zwart A, van Kampen EJ, Zizlstra WG. Results of routine determination of clinically significant hemoglobin derivatives by multicomponent analysis. Clin Chem 1986 32 972-8. 

The light absorption spectrum of the hemoglobin derivative to be chosen for a photometric determination should have a number of favorable properties. It should display a rather flat maximum. If this is not the case, slight alterations in filter or in photometer characteristics will introduce considerable errors. Furthermore, the light absorption maximum should... 

Because measurements dependent upon time always disturb laboratory routine, the hemoglobin derivative to be used for the determination of the Hb concentration in blood must be formed rapidly, all hemoglobins present in the sample must be converted, and the end product formed must be stable. Stability is an essential requisite for the use of standard solutions to calibrate the photometer to be used. The measurement itself should not be disturbed by plasma proteins (globulins) or erythroc3rte stromata, either through the introduction of a so-called protein error (C3) or because of turbidity. Only HiCN properly prepared fulfills all requirements mentioned. 

Assuming Lambert-Beer s law to be valid for all solutions over the entire spectral region, can be calculated from for any hemoglobin derivative, when the total hemoglobin concentration of the sample has been determined ... 

In the determination of the quarter millimolar extinction coefficients of hemoglobin derivatives, the following sources of error are present ... 

It is clear that in this case three constants must be determined. If the components 1 and 2 are used only for calibration, extreme care must be taken, when converting 1 to 2, that the total concentration is not altered in any way. If this is not the case, dangerous errors will be made in the determination of the constants B and C, for replacement of and D in Eq. (10) is allowed only when this requirement has been fulfilled. When use is made of the e values for hemoglobin derivatives (given in Section 3) to overcome this difficulty, one must be certain that measurement of the D values of the sample is performed under exactly the same conditions as those under which the e values were determined. 

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