Heme oxygenase expression

Heme oxygenase expression. Mainstream smoke trapped in phosphate-buffered saline solutions, in Swiss albino 3T3 fibroblasts, produced dose-dependent and transiently elevated expression of heme oxygenase. Heme oxygenase protein and its mRNA were detectable between 1 and 24 hours after exposure to 0.03 puffs (approx 1 cm / mL of medium). A nearly 50-fold increase in the amount of heme oxygenase mRNA was determined after 8 hours of exposure, compared to control levels. A decrease of more than 60% in glutathione levels was observed after the exposure. No elevated amounts of heme oxygenase mRNA appeared in smoke treated-cells when cysteine was exogenously added . 

NT083 Muller, T., and S. Gebel. Heme oxygenase expression in Swiss 3T3 cells following exposure to aqueous cigarette smoke fractions. Carcinogenesis 1994 15(1) 67-72. 

Ye, J. and Laychock, S. G. (1998). A protective role for heme oxygenase expression in pancreatic islets exposed to interleukin-lbeta. Endocrinology 139, 4155-4163. 

Rezzani R, Rodella L, Bianchi R, Goodman Al, Lianos EA. Protective effects of heme-oxygenase expression in cyclosporine A-in-duced injury. Curr Neurovasc Res 2005 2 157-1561. 

Kutty G, Hayden B, Osawa Y, Wiggert B, Chader GJ, Kutty RK. Heme oxygenase expression in human retina and modulation by stress agents in human retinoblastoma cell model system. Curr Eye Res 1992 11 153-160. 

Jeong, G.S. et al.. Comparative effects of curcuminoids on endothelial heme oxygeanse-1 expression ortho-methoxy groups are essential to enhance heme oxygenase activity and protection, Exp. Molec. Med., 38, 393, 2006. 

Wilks A, MP Schmitt (1998) Expression and characterization of a heme oxygenase (Hmu O) from Corynebacterium diphtheriae. J Biol Chem 2iy. 837-841. 

Obermuller-Jevic, UC. Schlegel, B. Flaccus, A., and Biesalski, HK. 2001. The effect of beta-carotene on the expression of interleukin-6 and heme oxygenase-1 in UV-irradiated human skin fibroblasts in vitro. FEBS Lett 509 186-190. 

Heme oxygenase HO-2 has been partially purified from rat testes and the full-length protein has been expressed in an active form in E. coli (11, 61-63). Truncation of the protein at the C-terminus provides... 

Mutation of His 132 to an alanine, glycine, or serine in the truncated human heme oxygenase, in accord with identification of His25 as the proximal ligand, does not alter the cpe-His band in the resonance Raman spectrum of the Fe deoxy protein (82). The mutations result in the formation of two separate fractions when the protein is expressed in... 

A dipeptide Met- , derived from sardine muscle (Matsufuji et ah, 1994), stimulates expression of the antioxidant defense protein HO-1 in a concentration-dependent manner. Previous findings revealed that HO-1 protein expression is accompanied by the induction of a secondary antioxidant protein, ferritin. In a present study, the effect of Met- on the expression of the antioxidant stress proteins, heme oxygenase-1 (HO-1), and ferritin in endothelial cells derived from the human umbilical vein and their contribution to the decrease in radical formation that occurs under the influence of this dipeptide were studied and reported potential activity (Erdmann et ah, 2006). 

Chow JM, Shen SC, Huan SK, Lin HY, Chen YC. 2005. Quercetin, but not rutin and quercitrin, prevention of H202-induced apoptosis via anti-oxidant activity and heme oxygenase 1 gene expression in macrophages. Biochem Pharmacol 69 1839-1851. 

Chen CY, Jang JH, Li MH, Surh YJ. 2005. Resveratrol upregulates heme oxygenase-1 expression via activation of NF-E2-related factor 2 in PC 12 cells. Biochem Biophys Res Commun 331 993-1000. 

Pugazhenthi S, Akhov L, Selvaraj G, Wang M, Alam J. 2007. Regulation of heme oxygenase-1 expression by demethoxy curcuminoids through Nrf2 by a PI3-kinase/... 

Hara E., Takahashi K., Tominaga T., Kumabe T., Kayama T., Suzuki H., Fujita H., Yoshi-moto T., Shirato K., and Shibahara S. (1996). Expression of heme oxygenase and inducible nitric oxide synthase mRNA in human brain tumors. Biochem. Biophys. Res. Commun. 224 153-158. 

Schipper H. M., Cisse S., and Stopa E. G. (1995). Expression of heme oxygenase-1 in the senescent and Alzheimer-diseased brain. Ann. Neurol. 37 758-768. 

Nakayama M, Takahashi K, Komura T, et al, Increased expression of heme oxygenase-1 and bilirubin accumulation in foam cells of rabbit atherosclerotic lesions. Arterioscler Thromb Vase Biol 2001 21(8) 1373-1377. 

Inhibits expression of vascular cell adhesion molecule-1 and E-selectin independently of heme oxygenase-1 expression [155]... 

Two genetically distinct heme oxygenases (HOs) have been characterized HO-1 is an inducible form, whereas HO-2 is constitutive. HO-2 is abundant in selective tissues such as brain and the cardiovascular system. HO-1 is highly inducible in many tissues in response to a variety of stresses, including infection, exposure to xenobiotics, hypoxia, and proinflammatory cytokines. The recognition that heme oxygenase is expressed in many different tissues led to studies of the role of heme degradation apart from the pathway for formation and elimination of bilirubin derived from destruction of senescent erythrocytes. 

The widespread expression of biliverdin reductase, which parallels the widespread expression of heme oxygenase, suggests that bilirubin may play a special role in particular tissues. Studies suggested that bilirubin is an excellent lipid-soluble antioxidant. Bilirubin reacts with reactive oxygen species (ROS) that oxidize bilirubin back to biliverdin. The biliverdin then is reduced again to bilirubin. This repeated cycling results in the net use of NADPH to protect cells from ROS (Fig. 22-1). This system may be especially important in neurons. 

Srisook, Klaokwan Cha YN Biphasie induetion of heme oxygenase-1 expression in macrophages stimulated with lipopolysaeeharide. Biochem Pharmacol, No. 68 1709-1720 2004. 

Liang D-Y, Li X, Clark JD (2004) Formalin-induced spinal cord calcium/cahnodulin-dependent protein kinase Ila expression is modulated by heme oxygenase in mice. Neurosci Lett 360 61-64... 

Zamora R, Vodovotz Y, Aultik KS, Kim PK, Kane JM 3rd, Alarcon L, Stuehr DJ, BiUiar TR (2002) A DNA microarray study of nitric oxide-induced genes in mouse hepatocytes imphcations for hepatic heme oxygenase-1 expression in ischemia/reperfusion. Nitric Oxide 7 165-186... 

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