Heavy chains genes producing

Fig. 4. Somatic recombination to create a heavy chain gene and expression of that gene to produce heavy chain polypeptides. For simplicity, the constant regions for the various Cy subclasses are not shown.

Abciximab (ReoPro) was the first partially humanized antibody approved for therapeutic use. It was produced from transgenic murine melanoma cells containing human heavy-chain genes so that the resulting Fab fragment is a chimera of human and mouse Ab fragments. Abciximab binds to the glycoprotein llb/llla... 

In another study to produce fluorescent silk, fibroin heavy chain gene replacement in silkworm through site-directed homologous recombination was carried out [48]. 

Somatic hypermutation Mutations occurring in the variable region genes of the light and heavy chains during the formation of memory B cells. Those B cells whose affinity is increased by such mutations are positively selected by interaction with antigen, and this leads to an increase in the average affinity of the antibodies produced. 

Fig. 1. Structure of the antibody fusion protein. The Fab-like form is formed from a X light chain derived from the J558L cell line, and the chimenc heavy chain produced from the vector. The heavy chain consists of a variable region with NIP binding activity, CH1, hinge and part of the CH2 domain of murine y2b heavy chain, and the foreign gene. The fusion protein may be secreted in this form or as an F(ab )2-like form consisting of 2 Fab-like molecules linked at the hinge region by three disulfide bonds...

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