Haemophilus influenzae capsular

FIGURE 7.8 Structure of the conserved inner core oligosaccharide regions of LPS from N. meningitidis and H. influenzae. (A) Haemophilus influenzae capsular and noncapsular strains. (B) Neisseria meningitidis strain. (Adapted from Li, J. et al. Methods Enzymol, 405, 369, 2005. With permission.)... 

In the cell-wall antigen from Staphylococcus aureus M, taurine is linked as an amide (51) to a 2-acetamido-2-deoxy-D-galactosyluronic residue. l-Threonine and L-glutamic acid are linked as amides to D-glucuronic acid residues in the LPS from Rhodopseudomonas sphaeroides ATCC 17023 and in the capsular polysaccharide from Klebsiella K82, respectively. In the capsular polysaccharide from E. coli K54, L-serine and L-threonine, in the ratio 1 9, are linked to the carboxyl group of a D-glucuronic acid residue. In the capsular polysaccharide from Haemophilus influenzae type d,... 

In different polysaccharides of the teichoic acid type, monosaccharides or oligosaccharides are connected by phosphoric diester linkages. Two examples are the capsular antigens from Neisseria meningitides type A (56) and Haemophilus influenzae type c (57), respectively. Glycerol phosphate... 

Haemophilus influenzae type b Cultures of H. influenzae type b 1 Separation of capsular polysaccharide 2 Conjugation with a protein Estimation of capsular polysaccharide content ... 

Anderson, P.W., Pichichero, M.E., Stein, E.C., Porcelli, S., Betts, R.F., Connuck, D.M., Korones, D., Insel, R.A., Zahradnik, J.M., and Eby, R. (1989) Effect of oligosaccharide chain length, exposed terminal group, and hapten loading on the antibody response of human adults and infants to vaccines consisting of Haemophilus influenzae type b capsular antigen uniterminally coupled to the diphtheria protein CRMI97./. Immunol. 142, 2464-2468. 

A further type of vaccine included in the subunit category is the capsular polysaccharide vaccines, for example, those against Haemophilus influenzae and meningococcal meningitis. In this case an extract of the polysaccharide outer capsule of the bacterium is used as a vaccine and is sometimes conjugated to protein to... 

Haemophilus influenzae immunoglobulins Human Antibodies raised against surface capsular polysaccharide of Haemophilus influenzae... 

Haemophilus influenzae Purified capsular polysaccharide of Active immunization against... 

Despite their popularity, aluminium-based adjuvants suffer from several drawbacks. They tend to effectively stimulate only the humoral arm of the immune response. They cannot be frozen or lyophylized, as either process promotes destruction of their gel-based structure. In addition, aluminium-based products display poor or no adjuvanticity when combined with some antigens (e.g. typhoid or Haemophilus influenzae type b capsular polysaccharides). 

Kayhty, H., V. Karanko, H. Peltola, and P.H. Makela, Serum antibodies after vaccination with Haemophilus influenzae type b capsular polysaccharide and responses to reimmunization no evidence of immunologic tolerance or memory. Pediatrics, 1984. 74(5) 857-65. 

Haemophilus influenzae is a Gram-negative human pathogen that routinely colonizes the upper respiratory tract. Serotype b capsular strains are associated with invasive diseases such as meningitis, septicemia, epiglottises, pneumonia, and emphysema, particularly in infants (1,2). The presence of TV-acetylglucos-amine as a minor component of lipopolysaccharide (LPS) has been reported in H. influenzae type b strain A2 (3) and in the related species Haemophilus ducreyi... 

In a programme directed towards the synthesis and later antigenic evaluation of all the six different capsular types of Haemophilus influenzae (type a-f), the development of an effective synthesis of fructofuranosides became necessary, since this is a motif in the type e structure [97] (Fig. 7). The synthesis of the repeating unit without the fructofuranosyl residue has been reported [98]. 

Kanra, G. Yurdakok, K. Ceyhan, M. Ozmert, E. Turkay, F. Pehlivan, T. Immunogenicity and safety of haemophilus influenzae type B capsular polysaccharide tetanus conjugate vaccine (PRP-T) presented in a dualchamber syringe with DTP. Acta Paediatr. Jpn. 1997, 39, 676-680. 

Capsular polysaccharide of bacteria is one of the significant targets for development of vaccines [128]. For example, Haemophilus influenza type B (Hib) vaccine, made of a polysaccharide-protein conjugate, is now used worldwide as a highly effective therapy [129]. Verez-... 

Contains 5 meg 0.5 ml HBsAg and 7.5 meg Haemophilus influenzae type B purified capsular polysaccharide fragments conjugated to 125 meg Neisseria meningitidisouler membrane protein complex. 

Fructose has also been found in the capsular polysaccharides of strains of Haemophilus influenza. The ketose is present as (3-D-fructofuranosyl residues in the repeating units.140... 

P. Branefors-Helander, Structural studies of two capsular polysaccharides elaborated by strains of Haemophilus influenza type e, Carbohydr. Res., 88 (1981) 77-84. 

Fully methylated and fully acetylated disaccharides isolated from the capsular polysaccharide of Escherichia coli KlOO and Haemophilus influenzae type b have been identified by m.s. as 2-0- and 1-O-D-ribofuranosyl ribitol, respectively. " The capsular antigen of H. influenzae type c has been purified by ion-exchange chromatography. Structural investigations have identified the disaccharide (8) as the repeating unit of the polysaccharide. 

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