Oligosaccharides chain length

Anderson, P.W., Pichichero, M.E., Stein, E.C., Porcelli, S., Betts, R.F., Connuck, D.M., Korones, D., Insel, R.A., Zahradnik, J.M., and Eby, R. (1989) Effect of oligosaccharide chain length, exposed terminal group, and hapten loading on the antibody response of human adults and infants to vaccines consisting of Haemophilus influenzae type b capsular antigen uniterminally coupled to the diphtheria protein CRMI97./. Immunol. 142, 2464-2468. 

Preliminary kinetic studies indicate that X4 hydrolysis was extremely slow, while larger straight-chain substrates were cleaved at rates that increased with increasing chain length. It is possible that branched-chain xylo-oligosaccharides were formed during incubation with Xylanase II their rates of formation and hydrolysis are as yet unknown. 

The difference in action patterns between these p-mannanases is shown clearly by fractionating and characterising the reaction products (Table I) (5). These products are a consequence of the abihty of the enzyme to cleave in the vicinity of D-mannosyl residues substituted by D-galactose, as well as the length of the 1,4-P-D-manno-oligosaccharide chain required by the enzyme for binding. The favoured conformation of the (l-4)-p-D-linked mannan chain is a flat... 

Oligosaccharides ranging from two to six repeating units were prepared by testicular hyaluronidase digestion of hyaluronate, and isolated by Sephadex G-50 chromatography. Experiments were carried out to determine the minimum chain length of hyaluronate to which core molecules would bind. When core molecules were mixed... 

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