Guanyl nucleotide receptor

Battaglia, G. Shannon, M. and Titeler. M. Guanyl nucleotide and divalent cation regulation of cortical S2 serotonin receptors. J Neurochem 43 1213-1219, 1984. 

Ratomponirina, C., Hode, Y., Hechler, V., and Maitre, M. (1995) Gamma-hydroxybutyrate receptor binding in rat brain is inhibited by guanyl nucleotides and pertussis toxin. Neuro sci. Lett. 189, 51-53. 

Initial biochemical studies indicate that agonist binding was regulated by guanyl nucleotides, implying that the receptor belongs to the superfamily of receptors coupled to G proteins. In addition, various intracellular responses were found to be associated with Hi-receptor stimulation inositol phosphate release, increase in Ca2+ fluxes, cyclic AMP or cyclic GMP accumulation in whole cells and arachidonic acid release [1],... 

Hill DR, Bowery NG, Hudson AL (1984) Inhibition of GABAb receptor binding by guanyl nucleotides. J Neurochem 42 652-7... 

Lyon RA, Davis KH, Titeler M. 3H-DOB (4-bromo-2,5-dimethoxyphenyl-isopropylamine) labels a guanyl nucleotide-sensitive state of cortical 5-HT2 receptors. Mol Pharmacol 1987 31 194-199. 

Adenylate cyclase is a two-component enzyme system. It ultimately catalyzes the cyclase reaction, but only when it is associated with the hormone-bound receptor and a regulatory protein called a stimulatory G-protein (guanylate nucleotide binding protein), which activates adenylate cyclase. The G-protein is the intermediate between the receptor and the synthesis of cyclic AMP. 

Snyder SH, Childers SR, Creese I. Molecular actions of opiates historical overview and new findings on opiate receptor interactions with enkephalins and guanyl nucleotides. Adv Biochem Psychopharmacol 1979 20 543-552. 

Perrin MH, Haas Y, Rivier JE, Vale WW (1986) Corticotropinreleasing factor binding to the anterior pituitary receptor is modulated by divalent cations and guanyl nucleotides. Endocrinology 118 1171-9... 

In the G protein s unactivated state, the guanyl nucleotide bound to the G protein is GDP. In this form, the G protein exists as a heterotrimer consisting of a, p, and y subunits the ct subunit (referred to as G ) binds the nucleotide (Figure 14.6). The a subunit is a member of the P-loop NTPase family (Section 9.4), and the P-loop participates in nucleotide binding. The a and y subunits are usually anchored to the membrane by covalently attached fatty acids. The role of the hormone-hound receptor is to catalyze the exchange of GTP for hound GDP. The hormone—receptor complex interacts with the heterotrimeric G protein and opens the nucleotide-hinding site... 

G-protein receptors are so named because their action is linked to the binding of guanyl nucleotides. 

Morphine-like peptides their regulation in the neuroendocrine system and the effect of guanyl nucleotides and divalent ions on opiate receptor binding... 

Locate guanyl nucleotide-binding proteins (G proteins) in the cell and describe their structures, catalytic characteristics, and molecular mechanisms of activation and inactivation. Describe the roles of G proteins in coupling a hormone-receptor complex to adenylate cyclase and in amplifying the stimulus. 

The main signal transduction mechanism for PTH receptor stimulation is the cAMP system. Stimulation of renal or bone PTH receptors causes an elevation of membrane-bound and guanyl nucleotide-dependent adenylate cyclase activity resulting in the enhanced formation of cAMP from ATP. Recent studies suggest that PTH may also stimulate inositol triphosphate and diacylglycerol production in renal tissue (7). 

Jesaitis, A.J., Bokoch, G.M., Tolley, J.O. and AUen, R.A. (1988). Lateral segregation of neutrophil chemoattractant receptors into actin- and fodrin-rich plasma membrane microdomains depleted in guanyl nucleotide regulatory proteins. ]. Cell. Biol. 107, 921-928. 

A number of experiments were undertaken to investigate whether there was altered coupling between the receptor (R), the guanyl-nucleotide regulatory binding protein or the catalytic subunit of adenylate cyclase (E) of... 

The receptors (R) interact with a complex (GE) of the adenylate cyclase catalytic subunit (E) and the guanyl-nucleotide binding regulatory protein (G) with a 1 1 stoichiometry and (t/) complexes of GE are activated when an equal number (y) of receptors are occupied. 

Figure 8.27 Arrangement of the constituent proteins in the lipid bilayer of a human platelet. The diagram shows a schematic representation of the receptors (R) for PGI2 and adenosine (AJ, the catalytic subunit of adenylate cyclase (E), and the 3 subunits (a, jS and y) of the guanyl nucleotide-binding regulatory protein (GJ...

Guillon, G., Butlen, D. and Rajerison, R. (1984). Evidence for two molecular forms of solubilized vasopressin receptors in rat kidney membranes Regulation by guanyl nucleotides. Mol Pharmacol, 26, 241-247... 

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