Golgi stained neuron

Bolam JP, Somogyi P, Totterdell S, Smith AD (1981) A second type of striatonigral neuron a comparison between retrogradely labelled and Golgi-stained neurons at the light and electron microscopic levels. 

Figure 17. Golgi-stained neuron three-dimensional display of a confocal data set acquired in reflection Color depth coding visualizes the three-dimensional neuron various spines of different size give a brushlike impression. (Specimen courtesy of B. Fori MANN. Institut fiir Er-niihrungswissenschaften. GieBen, Germany)...

Golgi, in early neuroanatomical studies (1898) staining neurones by silver impregnation, observed a reticular apparatus which was crescent shaped and appeared to be linked through canaliculi. The structure was also seen in secretory cells. Between 1949 and 1954, Baker reported the presence of similar systems in unfixed cells examined by phase contrast. The structures could be stained by osmium tetroxide and probably contained lipid. They also stained for glycoprotein and alkaline phosphatase. Baker s confirmation of the existence of the... 

Bolam JP, Clarke DJ, Smith AD, Somogyi P (1983) A type of aspiny neuron in the rat neostriatum aecumu-lates [ Hjgamma-aminobutyric acid combination of Golgi-staining, autoradiography, and electron microscopy. J. Comp. Neurol, 213, 121-134. 

Immediately deep to the glomeruli is a layer with a relatively low cell density but a very dense neuropil, the external plexiform layer (EPL). Golgi-stained sections reveal that the predominant neural elements in this layer are the dendrites of mitral/tufted and granule cells. The principal neuron types in EPL are external, middle (Fig. 12B) and deep tufted cells, named according to their relative depth in EPL, and the Van Ge-huchten cells. 

Italian physician Camillo Golgi (1843-1926) develops a staining technique that vastly improves researchers ability to see and study neurons. 

Figure 3.4. Cellular components of the central nervous system. (A) Neurons impregnated with Golgi silver satin, (C) Calbindin immunoreactive intemeurons in the neocortex, (G) astrocytes immunoreactive with an antibody against GFAP, (H) peri-vascular astrocytes components of the BBB, (I) oligodendrocytes and white matter tracts stained with luxol fast blue, (F) ependimal cells around the periventricular zone.

Fig. 30. A. Frontal section through the cerebellum and attached brainstem of an adult rat. All the Purkinje cells are stained by cyclic 3, 5 -guanosine monophosphate-dependent protein kinase (cGK) antiserum, including their dendrites in the molecular layer and their axon terminals in the deep nuclei and in the brainstem (arrow). Bar = 1 mm. B. Higher magnification of the neurons indicated by an arrow head in A. Like a few other isolated labelled cells found in variable locations, these cells are considered as ectopic Purkinje cells. Bar = 50 /tm. C. cGK immunoreactive neuron in the cerebellum of I day-old rat. This ectopic Purkinje cell is located in the white matter and its appearance mimics that of 1-day-old Purkinje cells as visualized in Golgi impregnated material. Bar = 25 /tm. Wassef and Sotelo (1984).

In an electron microscope study Shute and Lewis (1966) found most of the AChE to be presynaptic. Scattered local neurones (Golgi type II cells) in hilus fasciae den-tatae and stratum oriens stained for AChE. Terminals on the bodies of the pyramidal and granular cells, i.e., the inhibitory terminals of the basket cells, were as a rule devoid of AChE. Further, they observed that AChE-positive nerve terminals were less numerous than AChE-positive thin nerve fibres, and concluded that the pattern of AChE staining reflected the distribution of an AChE-containing preterminal nerve plexus rather than that of AChE-containing boutons. 

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