Golgi staining

Extensive studies have investigated the organisation and termination patterns of C-fibres, employing various techniques including Golgi staining, degeneration techniques... 

Bolam JP, Somogyi P, Totterdell S, Smith AD (1981) A second type of striatonigral neuron a comparison between retrogradely labelled and Golgi-stained neurons at the light and electron microscopic levels. 

Bolam JP, Clarke DJ, Smith AD, Somogyi P (1983) A type of aspiny neuron in the rat neostriatum aecumu-lates [ Hjgamma-aminobutyric acid combination of Golgi-staining, autoradiography, and electron microscopy. J. Comp. Neurol, 213, 121-134. 

Fig. 9. Calcium binding proteins in the MOB. A. Distribution of cells labeled with an antibody to Calbindin (CALB) using immunocytochemistry. B. Parvalbumin (PARV) immunocytochemically labeled cells are found mainly in the EPL in the bulb. C. Golgi stained section showing impregnated external tufted cells. D. An example of how cells may be identified by other methods. In this case an external tufted cell is shown after intracellular injection of the marker biocytin. Bar in D, /tm.

Immediately deep to the glomeruli is a layer with a relatively low cell density but a very dense neuropil, the external plexiform layer (EPL). Golgi-stained sections reveal that the predominant neural elements in this layer are the dendrites of mitral/tufted and granule cells. The principal neuron types in EPL are external, middle (Fig. 12B) and deep tufted cells, named according to their relative depth in EPL, and the Van Ge-huchten cells. 

FIGURE 6.11 GD30 labeled with antibody Ber-H2 in an anaplastic large cell lymphoma, T-cell, primary cutaneous type. Some cells exhibit paranuclear Golgi staining (center) in addition to strong membrane positivity. 

Figure 17. Golgi-stained neuron three-dimensional display of a confocal data set acquired in reflection Color depth coding visualizes the three-dimensional neuron various spines of different size give a brushlike impression. (Specimen courtesy of B. Fori MANN. Institut fiir Er-niihrungswissenschaften. GieBen, Germany)...

In 1882 Phillip Stohr of Wurzburg described vacuoles in oxyntic cells, but in 1898 Erik Muller of the Karolinska Institute in Stockholm demonstrated that the vacuoles are really parts of a canalicular system within the cells that communicates directly with the lumen of the gland s tubule or indirectly by means of a secretory capillary lying between chief cells. In Muller s Figure 6, the intracellular canaliculi of a rabbit s parietal cell are particularly well demonstrated by a Golgi stain."... 

Tamamaki N, Nakamma K, Furuta T et al (2000) Nemons in Golgi-stain-like images revealed by GFP-adenovirus infection in vivo. Neurosci Res 38 231-236... 

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