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Glycosaminoglycans analysis

Piraud M, Boyer M, Mathieu M, Maire I (1993) Diagnosis of mucopolysaccharidoses in a clinically selected population by urinary glycosaminoglycan analysis a study of 2000 urine samples. Clin Chim Acta 221 171-181... [Pg.324]

Although liquid chromatography (l.c.) under elevated pressure is now used essentially for determining the molecular weight of heparin,74,75 as an alternative to methods using soft gels,76 it can undoubtedly be used as well for rapid analysis of mixtures of glycosaminoglycans. [Pg.64]

Analysis of glycosaminoglycan hydrolyzates is often complicated by the presence of basic and acidic glycoses, in addition to neutral monosaccharides. Adsorption chromatography on silica gel was used to classify such a hydrolyzate, and the neutral fraction was shown to contain D-glucose, D-galactose, and D-mannose.288 Separation on ion-exchange resins may also be used.51,149 The resolution of the acidic and basic fractions is discussed in Sections IX (p. 71) and X (p. 78). [Pg.44]

Dembure PP, Roesel RA (1991) Screening for mucopolysaccharidoses by analysis of urinary glycosaminoglycans. In Hommes FA (ed) Techniques in Diagnostic Human Biochemical Genetics. Wiley-Liss, New York, pp 77-86... [Pg.322]

Kodama C (1988) Methods for analysis of urinary glycosaminoglycans. J Chromatogr 429 293-313... [Pg.323]

Mao W, Thanawiroon C, Linhardt RJ (2002) Capillary electrophoresis for the analysis of glycosaminoglycans and glycosaminoglycan-derived oligosaccharides. Biomed Chromatogr 16 77-94... [Pg.323]

Matsuno Y, Kinoshita M, Kakehi (2005) Fast analysis of glycosaminoglycans by microchip electrophoresis with in situ fluorescent detection using ethidium bromide. J Pharm Biomed Anal 37 429-436... [Pg.323]

Infrared spectra of glycosaminoglycans in D20 and DC1 were used165 for quantitative evaluation of the uronic acid and acetamido groups in this class of polysaccharide. The results showed that i.r. spectroscopy of compounds in D20 solution provides a simple, quantitative basis for examination of ras(COJ) and amide I bands in heparin and chondroitin 4-sulfate. In DC1, analysis was based on p(C02H) and amide I bands which were well resolved. The apparent acid-dissociation constants of the studied polysaccharides were estimated from the absorbance of p(C02) or p(C02H) bands, or both, at different pH (pD) values. [Pg.55]

Martin, I., Obradovic, B., Freed, L.E., and Vunjak-Novakovic, G. (1999) Method for quantitative analysis of glycosaminoglycan distribution in cultured natural and engineered cartilage. Ann. Biomed. Eng. 27, 656-662... [Pg.212]

Takahashi, Y. et al., Disaccharide analysis of human skin glycosaminoglycans in sun-exposed and sun-protected skin of aged people, J. Dermatol. Sci., 11, 129, 1996. [Pg.273]

Analysis of urine for excess substrates (e.g., glycosaminoglycans in the Mucopolysaccharidoses, globotriaolsylceramide in Fabry disease) may also suggest the presence of an LSD. In any case, all patients suspected to have an LSD should have diagnostic confirmation by means of biochemical and/or molecular genetic analysis. [Pg.791]

For the enzymic analysis of polysaccharide structure, the hydrolytic enzymes are the most useful, and little attention will be paid in this article to other types of enzyme. No consideration at all will be accorded enzymes acting on non-carbohydrate moieties. For diis reason, heteropolysaccharide-peptide and lipopolysaccharide complexes (such as those from bacterial cell-walls) are not discussed, as satisfactory treatment of these would also require mention of other types of enzyme, such as amidases for coverage of this subject, an authoritative text and several reviews - may be consulted. Other types of carbohydrate-containing macromolecule, such as glycosaminoglycans ( mucopolysaccharides ) and glycoproteins, for which it is relatively easy to restrict consideration to the polysaccharide portion, are discussed, but only with reference to their carbohydrate moieties. [Pg.259]

Transmission electron microscopy analysis revealed a marked expansion of the endomysial space in thyroid eye disease extraocular muscle biopsies compared with that in control biopsies (69). An increased number of collagen fibers with hyaluronan were detected by immunogold staining, although the serum levels of hyaluronan and urinary glycosaminoglycans were not found to be sensitive indicators for the presence of these molecules within the extraocular muscles (69). Imai et al. further confirmed that the local accumulation of glycosaminoglycans in thyroid eye disease was not associated with the serum hyaluronan concentration (70). [Pg.191]

The hyaluronan in the component of pseudoexfoliation material from human donor eyes has been analyzed histochemically. Hyaluronan was found to coat the fibrillar exfoliation material on the lens, zonules, iris epithelium, and ciliary body (85). The major component of the pseudoexfoliation material on the posterior surface of the iris was histochemically verified as chondroitin sulfate, while the minor component was hyaluronan (86). A polarization microscopic analysis demonstrated the presence of more sulfated glycosaminoglycans in exfoliation syndrome than in control subjects. [Pg.194]

Kara A, Nakagomi Y. Analysis of glycosaminoglycans of snbretinal fluid in rhegmatogenons retinal detachment-preliminary report. Jpn J Ophthalmol 1995 39 137-142. [Pg.203]

Pappa A, Jackson P, Stone J, Munro P, Fells P, Pennock C, Lightman S. An ultrastructural and systemic analysis of glycosaminoglycans in thyroid-associated ophthalmopathy. Eye 1998 12 237-244. [Pg.204]

Saito A, Munakata H. Analysis of plasma proteins that bind to glycosaminoglycans. Biochim Biophys Acta 2007 1770 241-624. [Pg.220]


See other pages where Glycosaminoglycans analysis is mentioned: [Pg.137]    [Pg.414]    [Pg.17]    [Pg.194]    [Pg.6]    [Pg.327]    [Pg.7]    [Pg.65]    [Pg.320]    [Pg.43]    [Pg.173]    [Pg.310]    [Pg.161]    [Pg.261]    [Pg.382]    [Pg.166]    [Pg.33]    [Pg.124]    [Pg.23]    [Pg.52]   
See also in sourсe #XX -- [ Pg.46 , Pg.55 ]




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